Biotin anti-human GM-CSF
Description
Granulocyte/macrophage - colony stimulating factor (GM-CSF) is a hematopoietic factor that is produced by activated T cells, B cells, mast cells, macrophages, fibroblasts, and endothelial cells. In addition to supporting colony formation of granulocyte/macrophage progenitors, GM-CSF is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of human GM-CSF protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut, IP)
2. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133. (ELISA)
3. Bacchetta R, et al. 1990. J. Immunol. 144:902. (ELISA)
4. Kita H, et al. 1991. J. Exp. Med. 174:745. (ELISA)
5. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
6. Andersson J, et al. 1994. Immunology 83:16. (IHC)
7. Rasouli J, et al. 2015. J. Immunol. 11:5085-93. (IHC)