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Applications

Key Extracellular Treg Markers

Key Extracellular Treg Markers
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD3 OKT3 Identification of T Cells PacBlue
(2186565, 2186570)BV510
(2186655, 2186660)BV605
(2186605, 2186610)

BV650
(2186615, 2186620)

BV711
(2186635, 2186640)

BV785
(2186645, 2186650)

 FITC
(2186525, 2186530)AF488
(2186550)PerCP
(2186685, 2186690)

PerCP/Cy5.5
(2186675, 2186680)

PE
(2186535, 2186540)

PE/Cy7
(2186665, 2186670)

 APC
(2186585, 2186590)AF647
(2186560)AF700
(2186695, 2186700)

APC/Cy7
(2186705, 2186710)
 UCHT1 Identification of T Cells PacBlue
(2102090, 2102085)BV421
(2102165, 2102170)BV510
(2102235, 2102240)

BV570
(2102175, 2102180)

BV605
(2102295, 2102300)

 FITC
(2102025, 2102030)AF488
(2102075)PerCP
(2102135, 2102140)

PerCP/Cy5.5
(2102145, 2102150)

PE
(2102035, 2102040)

PE/Dazzle594
(2102245, 2102250)

PE/Cy5
(2102050)

PE/Cy7
(2102095, 2102100)

 APC
(2102055, 2102060)AF647
(2102110, 2102080)AF700
(2102115, 2102120)

APC/Cy7
(2102125, 2102130)
CD4 RPA-T4 Identification of CD4+ helper T cells PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

 FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE/Dazzle594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560)

 APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585, 2102590)
CD25 BC96 Tregs express high levels of CD25 PacBlue
(2113135)BV421
(2113145, 2113150)BV605
(2113155, 2113160)

BV650
(2113165, 2113170)

BV711
(2113175, 2113180)

BV785
(2113185)

 FITC
(2113015, 2113020)AF488
(2113075, 2113080)PerCP/Cy5.5
(2113125, 2113130)

PE
(2113025, 2113030)

PE/Cy5
(2113035, 2113040)

PE/Cy7
(2113055, 2113060

 APC
(2113045, 2113050)AF647
(2113085, 2113090)AF700
(2113105, 2113110)

APC/Cy7
(2113065, 2113070)
 M-A251  PacBlue
(2380645, 2380650)BV421
(2380565, 2380570)BV510
(2380595, 2380600)		 FITC
(2380525, 2380530)AF488
(2380575, 2380580)PerCP
(2380655, 2380660)

PerCP/Cy5.5
(2380555, 2380560)

PE
(2380515, 2380520)

PE/Dazzle594
(2380625, 2380630)

PE/Cy7
(2380535, 2380540)

 APC
(2380545, 2380550)AF647
(2380635, 2380640)AF700
(2380585, 2380590)

APC/Cy7
(2380605, 2380610)
CD127 A019D5 Inversly correlates with FoxP3 expression. Useful for T-reg enrichment/isolation. PacBlue
(2356525, 2356530)BV421
(2356545, 2356550)BV510
(2356655, 2356660)

BV570
(2356535)

BV605
(2356665, 2356670)

BV650
(2356625, 2356630)

BV711
(2356635, 2356640)

BV785
(2356645, 2356650)

 FITC
(2356555, 2356560)AF488
(2356565, 2356570)PerCP/Cy5.5
(2356605, 2356610)

PE
(2356515, 2356520)

PE/Dazzle594
(2356675, 2356680)

PE/Cy5
(2356615, 2356620)

PE/Cy7
(2356595, 2356600)

 APC
(2356575, 2356580)AF647
(2356585, 2356590)

Key Intracellular Treg Markers

Other Important Treg Markers

Other Important Treg Markers
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD6 BL-CD6 CD6 low expression may be useful in combination with CD127 for Treg enrichment FITC
(2169520)PE
(2169530)
CD39 A1 Identifies a subset of Tregs. May be important for Treg function BV421
(2241065, 2241070)BV510
(2241095)		 FITC
(2241025, 2241030)PerCP/Cy5.5
(2241085, 2241090)PE
(2241035, 2241040)

PE/Dazzle594
(2241115, 2241120)

PE/Cy7
(2241055, 2241060)

 APC
(2241045, 2241050)
CD45RA HI100 Useful to distinguish nTreg versus iTreg PacBlue
(2120585, 2120590)BV421
(2120645, 2120650)BV510
(2120705, 2120710)

BV570
(2120655, 2120660)

BV605
(2120665, 2120670)

BV650
(2120675, 2120680)

BV711
(2120685, 2120690)

BV785
(2120695, 2120700)

 FITC
(2120525, 2120530)AF488
(2120570)PerCP/Cy5.5
(2120605, 2120610)

PE
(2120535, 2120540)

PE/Dazzle594
(2120725, 2120730)

PE/Cy5
(2120550)

PE/Cy7
(2120625, 2120630)

 APC
(2120555, 2120560)AF700
(2120595, 2120600)APC/Cy7
(2120635, 2120640)
CD45RO UCHL1 T-cell memory/differentiation marker PacBlue
(2121075, 2121080)BV421
(2121115, 2121120)BV570
(2121125, 2121130)

BV605
(2121185, 2121190)

BV650
(2121155, 2121160)

BV711
(2121175)

BV785
(2121165, 2121170)

 FITC
(2121020)AF488
(2121060)PerCP/Cy5.5
(2121105, 2121110)

PE
(2121025, 2121030)

PE/Cy5
(2121040)

PE/Cy7
(2121145, 2121150)

 APC
(2121050)AF700
(2121085, 2121090)APC/Cy7
(2121135, 2121140)
CD137 4B4-1 Activation marker. Recommended to be used in combination with CD154 BV421
(2149095, 2149100)		 PerCP/Cy5.5
(2149065, 2149070)PE
(2149015, 2149020)PE/Cy5
(2149040)

PE/Cy7
(2149085, 2149090)

 APC
(2149045, 2149050)AF700
(2149080)
CD154 24-31 Activation marker. Recommended to be used in combination with CD137 PacBlue
(2154095, 2154100)BV421
(2154115, 2154120)BV510
(2154145)

BV605
(2154125)

BV711
(2154185, 2154190)

 FITC
(2154020)AF488
(2154075)PerCP/Cy5.5
(2154165, 2154170)

PE
(2154025, 2154030)

PE/Cy5
(2154035, 2154040)

PE/Cy7
(2154155, 2154160)

 APC
(2154045, 2154050)AF647
(2154090)APC/Cy7
(2154105, 2154110)
CD183
(CXCR3)		 G025H7 Possible marker of activated Treg PacBlue
(2368615, 2368620)BV421
(2368575, 2368580)BV510
(2368625, 2368630)

BV605
(2368635, 2368640)

BV650
(2368645)

BV711
(2368655, 2368660)

 FITC
(2368515, 2368520)AF488
(2368545, 2368550)PerCP/Cy5.5
(2368565, 2368570)

PE
(2368525, 2368530)

PE/Dazzle594
(2368675, 2368680)

PE/Cy7
(2368595, 2368600)

 APC
(2368535, 2368540)AF647
(2368555, 2368560)APC/Cy7
(2368605, 2368610)
CD184
(CXCR4)		 12G5 Involved in Treg migration BV421
(2132585, 2132590)BV605
(2132605, 2132610)		 PerCP/Cy5.5
(2132575, 2132580)PE
(2132525, 2132530)PE/Dazzle594
(2132625, 2132630)

PE/Cy5
(2132535, 2132540)

PE/Cy7
(2132565, 2132570)

 APC
(2132545, 2132550)
CD194
(CCR4)		 L291H4 Involved in Treg trafficking; expressed on tumor infiltrating T cells BV421
(2397065, 2397070)BV510
(2397075, 2397080)BV605
(2397085, 2397090)		 PerCP/Cy5.5
(2397025, 2397030)PE
(2397055, 2397060)PE/Dazzle594
(2397095, 2397100)

PE/Cy7
(2397045, 2397050)

 APC
(2397035, 2397040)AF647
(2397015, 2397020)
 TG6/CCR4  PerCP/Cy5.5
(2277015)		 AF647
(2277005)
CD278
(ICOS)		 C398.4A May identify a Treg subset that expresses higher levels of IL-10 PacBlue
(2167605, 2167610)BV421
(2167615, 2167620)BV510
(2167625)		 FITC
(2167525, 2167530)AF488
(2167570)PerCP/Cy5.5
(2167585, 2167590)

PE
(2167535, 2167540)

PE/Cy7
(2167595, 2167600)

 APC
(2167545, 2167550)AF647
(2167575, 2167580)
CD279
(PD-1)		 EH12.2H7 May be expessed by tumor infiltrating T-reg PacBlue
(2249575, 2249580)BV421
(2249595, 2249600)BV510
(2249655, 2249660)

BV605
(2249615, 2249620)

BV711
(2249635, 2249640)

BV785
(2249645, 2249650)

 FITC
(2249515, 2249520)AF488
(2249675, 2249680)PerCP
(2249685, 2249690)

PerCP/Cy5.5
(2249565, 2249570)

PE
(2249525, 2249530)

PE/Dazzle594
(2249695, 2249700)

PE/Cy7
(2249585, 2249590)

 APC
(2249535, 2249540)AF647
(2249550)APC/Cy7
(2249605, 2249610)
Galectin-9 9M1-3 Reported to increase iTreg stability FITC
(2344555, 2344560)PerCP/Cy5.5
(2344545, 2344550)PE
(2344525, 2344530)		 APC
(2344535, 2344540)
GARP
(LRRC32)		 7B11 May be useful for the identification of antigen-specific T-regs BV421
(2362545)		 PE
(2362515, 2362520)PE/Cy7
(2362535, 2362540)		 APC
(2362525, 2362530
HLA-DR L243 Identifies a subset of Treg PacBlue
(2138115, 2138120)BV421
(2138175, 2138180)BV510
(2138225, 2138230)

BV570
(2138185)

BV605
(2138195, 2138200)

BV650
(2138245, 2138250)

BV711
(2138215, 2138220)

BV785
(2138205, 2138210)

 FITC
(2138015, 2138020)AF488
(2138095, 2138100)PerCP
(2138135, 2138140)

PerCP/Cy5.5
(2138145, 2138150)

PE
(2138025, 2138030)

PE/Dazzle594
(2138265, 2138270)

PE/Cy5
(2138035, 2138040)

PE/Cy7
(2138075, 2138080)

 APC
(2138045, 2138050)AF647
(2138105, 2138110)AF700
(2138125, 2138130)

APC/Cy7
(2138085, 2138090)
LAP
(TGF-β1)		 TW4-2F8 May be useful for the identification of antigen-specific T-regs BV421
(2348065)		 FITC
(2348025, 2348030)PerCP/Cy5.5
(2348055, 2348060)PE
(2348015, 2348020)

PE/Cy7
(2348050)

 APC
(2348035, 2348040)
Perforin B-D48 Important for Treg functions PacBlue
(2366525)BV421
(2366535)

Key Intracellular Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
IL-10 JES3-9D7 Key cytokine for T-reg but levels often below detection limits BV421
(3107105)		 AF488
(3107065, 3107055)PerCP/Cy5.5
(3107085, 3107090)PE
(3107015, 3107020)

PE/Cy
(73107095, 3107100)

 APC
(3107045, 3107050)AF647
(3107070, 3107060)

Th1 Key Markers

Th1 Key Markers
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD4 RPA-T4 Identification of CD4+ helper T cells PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

 FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE/Dazzle594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560)

 APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585 2102590)
CD183
(CXCR3)		 G025H7 Highly expressed on Th1 cells; trafficking PacBlue
(2368615, 2368620)BV421
(2368575, 2368580)BV510
(2368625, 2368630)

BV605
(2368635, 2368640)

BV650
(2368645)

BV711
(2368655, 2368660)

 FITC
(2368515, 2368520)AF488
(2368545, 2368550)PerCP/Cy5.5
(2368565, 2368570)

PE
(2368525, 2368530)

PE/Dazzle594
(2368675, 2368680)

E/Cy5Â PE/Cy7
(2368595, 2368600)

 APC
(2368535, 2368540)AF647
(2368555, 2368560)APC/Cy7
(2368605, 2368610)

Key Th1 Intracellular Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
IFN-γ 4S.B3 Th1 signature cytokine PacBlue
(3112605, 3112610)BV421
(3112655, 3112660)BV510
(3112715, 3112720)

BV570
(3112665, 3112670)

BV605
(3112675, 3112680)

BV650
(3112685, 3112690)

BV711
(3112695, 3112700)

BV785
(3112705, 3112710)

 FITC
(3112525, 3112530)AF488
(3112585, 3112575)PerCP/Cy5.5
(3112625, 3112630)

PE
(3112540, 3112545)

PE/Dazzle594
(3112725, 3112730)

PE/Cy7
(3112635, 3112640)

 APC
(3112555, 3112560)AF647
(3112580)AF700
(3112595, 3112600)

APC/Cy7
(3112645, 3112650)
IFN-γ B27 Â FITC
(3132520)PE
(3132530, 3132535)PE/Cy7
(3132585, 3132590)		 APC
(3132550)AF700
(3132575, 3132580)
LT-α
(TNF-β)		 359-81-11 Cytokine expressed by Th1 cells PE
(3115525)
TNF-α Mab11 Cytokine expressed by Th1 cells PacBlue
(3114600)BV421
(3114655, 3114660)BV605
(3114675, 3114680)

BV650
(3114685, 3114690)

BV711
(3114695)

 FITC
(3114530)AF488
(3114585, 3114575)PerCP/Cy5.5
(3114625, 3114630)

PE
(3114540, 3114545)

PE/Dazzle594
(3114725, 3114730)

PE/Cy7
(3114645, 3114650)

 APC
(3114560)AF647
(3114580)AF700
(3114635, 3114640)
T-bet 4B10 Key Th1 transcription factor PacBlue
(3824035, 3824040)BV421
(3824075(125µl),3824080(500µl))BV605
(3824085(125µl))

BV711
(3824095(125µl))

 FITC
(3824055, 3824060)PerCP/Cy5.5
(3824025, 3824030)PE
(3824045, 3824050)

PE/Cy7
(3824115, 3824120)

 APC
(3824065, 3824070)AF647
(3824015, 3824020)

Th2 Markers

Th2 Markers
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD4 RPA-T4 Identification of CD4+ helper T cells PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

 FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE/Dazzle594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560)

 APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585,2102590)
CD194
(CCR4)		 L291H4 Th2 homing BV421
(2397065, 2397070)BV510
(2397075, 2397080)BV605
(2397085, 2397090)		 PerCP/Cy5.5
(2397025, 2397030)PE
(2397055, 2397060)PE/Dazzle594
(2397095, 2397100)

PE/Cy7
(2397045, 2397050)

 APC
(2397035, 2397040)AF647
(2397015, 2397020)
CD294
(CRTH2)		 BM16 Th2 marker. May be important in inflammation. BV421
(2350555, 2350560)		 FITC
(2350535, 2350540)PerCP/Cy5.5
(2350575, 2350580)PE
(2350525, 2350530)

PE/Cy7
(2350585, 2350590)

 APC
(2350545, 2350550)AF647
(2350515, 2350520)APC/Cy7
(2350565, 2350570)

Intracellular Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
IL-4 8D4-8 Signature cytokine for Th2 cells AF488
(3103550)PE
(3103515, 3103520)		 APC
(3103565, 3103570)AF647
(3103560)
 MP4-25D2  BV421
(3104125)BV605
(3104135, 3104140)		 FITC
(3104030)AF488
(3104085)PerCP/Cy5.5
(3104105, 3104110)

PE
(3104040)

PE/Dazzle594
(3104155, 3104160)

PE/Cy7
(3104115, 3104120)

 APC
(3104055, 3104060)AF647
(3104090)
IL-5 TRFK5 Expressed on a subset of Th2 cells BV421
(3121555(50ug))		 PE
(3121515, 3121520)		 APC
(3121525, 3121530)
IL-13 JES10-5A2 Cytokine expressed by Th2 cells PerCP/Cy5.5
(3109555)PE
(3109515)		 APC
(3109535)
GATA3 16E10A23 Key transcription factor for Th2 cells BV421
(3869065, 3869070)		 AF488
(3869035, 3869040)PerCP/Cy5.5
(3869055, 3869060)PE
(3869020)		 APC
(3869025, 3869030)AF647
(3869045, 3869050)

Th9 Markers

Th9 Markers
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD4 RPA-T4 Identification of CD4+ helper T cells PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

 FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE/Dazzle594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560)

 APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585, 2102590)

Intracellular Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
IL-9 MH9A4 Signature cytokine for Th9 cells PE
(3138020, 3138025)		 AF647
(3138035, 3138040)
BATF 9B5A13 Transcription factor expressed by Th9 cells PE
(3874015, 3874020)
IRF4 IRF4.3E4 Transcription factor expressed by Th9 cells AF488
(3832025, 3832030)PE
(3832015, 3832020)		 AF647
(3832035, 3832040)
SPI1
(PU.1)		 7C6B05 Transcription factor expressed by Th9 cells AF647
(3890015, 3890020)

Th17 Markers

Th17 Markers
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD4 RPA-T4 Identification of CD4+ helper T cells PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

 FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE/Dazzle594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560

 APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585, 2102590)
CD196
(CCR6)		 G034E3 Â BV421
(2367035, 2367040)BV510
(2367115)BV605
(2367095, 2367100)

BV650
(2367125, 2367130)

BV785
(2367105, 2367110)

 FITC
(2367055, 2367060)AF488
(2367065, 2367070)PerCP/Cy5.5
(2367025, 2367030)

PE
(2367045, 2367050)

PE/Dazzle594
(2367145, 2367150)

PE/Cy7
(2367085, 2367090)

 APC
(2367075, 2367080)AF647
(2367015, 2367020)
CD39 A1 Identifies a subset of Th17 cells BV421
(2241065, 2241070)BV510
(2241095)		 FITC
(2241025, 2241030)PerCP/Cy5.5
(2241085, 2241090)PE
(2241035, 2241040)

PE/Dazzle594
(2241115, 2241120)

PE/Cy7
(2241055, 2241060)

 APC
(2241045, 2241050)
CD73 AD2 Expressed by a subset of Th cells PacBlue
(Biotin only)BV421
(2320085)
CD161 HP-3G10 Expressed on most Th17 cells PacBlue
(2299625, 2299630)BV421
(2299565, 2299570)BV510
(2299605, 2299610)

BV570Â BV605
(2299575, 2299580)

BV711Â BV785
(2299645, 2299650)

 FITC
(2299525, 2299530)AF488
(2299615, 2299620)PerCP
(2299665, 2299670)

PerCP/Cy5.5
(2299535, 2299540)

PE
(2299515, 2299520)

PE/Cy7
(2299585, 2299590)

 APC
(2299555, 2299560)AF647
(2299545, 2299550)APC/Cy7
(2299635, 2299640)Â

Intracellular Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
IL-17A BL168 Signature Th17 cytokine PacBlue
(3161555, 3161560)BV421
(3161605, 3161610)BV510
(3161645, 3161650)

BV570
(2356515, 2356520)

BV605
(3161625, 3161630)

BV711
(3161635, 3161640)

 FITC
(3161515, 3161520)AF488
(3161535, 3161540)PerCP/Cy5.5
(3161565, 3161570)

PE
(3161525, 3161530)

PE/Dazzle594
(3161675, 3161680)

PE/Cy7
(3161575)

 APC
(3161665, 3161670)AF647
(3161545, 3161550)AF700
(3161585, 3161590)

APC/Cy7
(3161595, 3161600)
IL-21 3A3-N2 Â PE
(3165015, 3165020)		 APC
(3165035, 3165040)AF647
(3165025, 3165030)
TNF-α Mab11 Cytokine expressed by Th1 cells PacBlue
(3114600)BV421
(3114655, 3114660)BV605
(3114675, 3114680)

BV650
(3114685, 3114690)

BV711
(3114695)

 FITC3114530)

AF488
(3114585, 3114575)

PerCP/Cy5.5
(3114625, 3114630)

PE
(3114540, 3114545)

PE/Dazzle594
(3114725, 3114730)

PE/Cy7
(3114645, 3114650)

 APC
(3114560)AF647
(3114580)AF700
(3114635, 3114640)

APC/Cy7
(3114715, 3114720)

Th22 Cells

Th22 Cells
Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD4 RPA-T4 Identification of CD4+ helper T cells PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

 FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE/Dazzle594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560)

 APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585, 2102590)
CD194
(CCR4)		 L291H4 Homing receptor expressed on Th22 cells BV421
(2397065, 2397070)BV510
(2397075, 2397080)BV605
(2397085, 2397090)		 PerCP/Cy5.5
(2397025, 2397030)PE
(2397055, 2397060)PE/Dazzle594
(2397095, 2397100)

PE/Cy7
(2397045, 2397050)

 APC
(2397035, 2397040)AF647
(2397015, 2397020)
CD196
(CCR6)		 G034E3 Homing receptor expressed on Th22 cells BV421
(2367035, 2367040)BV510
(2367115)BV605
(2367095, 2367100)

BV650
(2367125, 2367130)

BV785
(2367105, 2367110)

 FITC
(2367055, 2367060)AF488
(2367065, 2367070)PerCP/Cy5.5
(2367025, 2367030)

PE
(2367045, 2367050)

PE/Dazzle594
(2367145, 2367150)

PE/Cy7
(2367085, 2367090)

 APC
(2367075, 2367080)AF647
(2367015, 2367020)
CCR10 6588-5 Homing receptor expressed on Th22 cells PE
(2307515, 2307520)		 APC
(2307525, 2307530)

Intracellular Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
IL-22 BG/IL-22 PE
(3176515)

T-Cell Panels

These panels are based on panels that have been published. Additional markers may be added for more information. The first panel is completely extracellular and is therefore suitable for cell sorting and analysis applications. The second one contains FoxP3, an intracellular marker, and is therefore only suitable for the analysis of Tregs. WeÂ’'ve designed these panels, based on published panels and included the references below.

This table is completely extracellular and is therefore suitable for cell sorting and analysis applications
Specificity Clone Fluorochrome Purpose Cat. No. Name
CD25 M-A251 PE T cell activation/Treg ID 2380515 PE anti-Human CD25
CD4 SK3 PerCP-Cy5.5 T cell subset 2323035 PerCP/Cy5.5 anti-human CD4
CD194 (CCR4) L291H4 PE/Cy7 T cell trafficking 2397045 PE/Cy7 anti-human CD194 (CCR4)
CD127 (IL-7Rα) A019D5 Alexa Fluor® 647 Low expession is correlated with Treg 2356585 Alexa Fluor® 647 anti-human CD127 (IL-7Rα)
CD45RO UCHL1 APC/Cy7 Maturation/Differentiation 2121135 APC/Cy7 anti-human CD45RO
CD3 UCHL1 Pacific Blueâ„¢ T cell marker 2102090 Pacific Blueâ„¢ anti-human CD3
HLA-DR LN3 FITC Treg Subset 2235025 FITC anti-human HLA-DR
This table is contains FoxP3, an intracellular marker, and is therefore only suitable for the analysis of Tregs
Specificity Clone Fluorochrome Purpose Cat. No. Name
CD3 UCHT1 FITC T cell marker 2102030 FITC anti-human CD3
CD4 SK3 PerCP-Cy5.5 2323035 PerCP/Cy5.5 anti-human CD4
CD45RO UCHL1 Brilliant Violet 421â„¢ Maturation Differentiation 2121115 Brilliant Violet 421â„¢ anti-human CD45RO
CD25 M-A251 PE/Dazzleâ„¢ 594 T cell activation/Treg ID 280625 PE/Dazzleâ„¢ 594 anti-human CD25
FOXP3 206D PE Treg Subsets 2200535 PE anti-human FOXP3
CD39 A1 PE-Cy7 2241055 PE/Cy7 anti-human CD39
CD49d 9F10 PE-Cy5 2121530 PE/Cy5 anti-human CD49d

References: http://iti.stanford.edu/himc/flow-cytometry.html

Murdoch, David M., Janet S. Staats, and Kent J. Weinhold. "OMIP‐006: Phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry." Cytometry Part A 81.4 (2012): 281-283.

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