APC/Fire™ 750 anti-mouse IgG2a

Secondary Antibody
Sony
RMG2a-62
Flow Cytometry
Rat IgG
Mouse
Mouse Ig cocktail
2635615
$92.00

Description

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG1-1 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

  1. Gavin AL, et al.2006. Science 314:1936.
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed