APC anti-human CD11b (activated)

Antibodies Single
Sony
CBRM1/5
Flow Cytometry
Mouse IgG1, κ
Human
2107050
$324.00

Description

The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block)
2. Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed
3. Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
4. Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed
5. Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed
6. Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed
7. Koleva RI, et al. 2012. Blood. 119:4878. PubMed.
8. Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed
9. Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215.
10. Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed
11. Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed