Anti-HA.11 Epitope Tag

Antibodies Single
Sony
16B12
Other
Mouse IgG1, κ
Other
Monoclonal antibody HA.11 (HA, 16B12, flu tag) was raised against the twelve amino acid peptide CYPYDVPDYASL.
5107575
$762.00

Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA. 

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is a dilution of 1:1000 – 1:5000 . For immunohistochemistry, the suggested use of this reagent is a dilution of 1:1,000 is suggested. For immunoprecipitation, the suggested use of this reagent is a dilution of 1:150 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Pecot MY, Malhotra V. 2004. Cell. 116:99.
2. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
3. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
4. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
5. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508.
6. Field J, et al. 1988. Mol Cell Biol. 8:2159.
7. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
8. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed