Alexa Fluor® 700 anti-human HLA-DR

Antibodies Single
Sony
LN3
Flow Cytometry
Mouse IgG2b, κ
Human
human PBL
2235070
$236.00

Description

The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Marder RJ, et al. 1985. Lab. Invest. 52:497.
2. Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.
3. Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441.