Alexa Fluor® 647 anti-mouse/human Mac-2 (Galectin-3)

Antibodies Single
Sony
M3/38
Flow Cytometry
Rat IgG2a, κ
Human,Mouse
Raised against galectin-3 of mouse origin
100 µg
1227040
$260.00

Description

Galectins, a family of carbohydrate binding proteins (lectins) have been implicated in inflammation and cancer. All galectins bind lactose and other β-galactosides but differ in their affinity for more complex saccharides.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 microg per million cells in 100 microL volume. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5-10 microg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

References

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2. Rosenberg I, et al. 1991. J. Biol. Chem. 266:18731. (WB, IP)
3. Evans CE, et al. 2010. Arterioscler Vasc Biol. (IHC) PubMed
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5. Li X, et al. 2011. Am J Physiol Heart Circ Physiol. 301:1932. (IHC) PubMed
6. Chao C, et al. 2012. Clin Cancer Res. 18:4702. (IHC) PubMed
7. Melo FH, et al. 2011. PLoS One. 6:e29313. (IF)
8. Usategui A, et al. 2013. Ann Rheum Dis. PubMed (IF)
9. Mey A, et al. 1996. J. Immunol. 156:1572. (ELISA)
10. Kouo T, et al. 2015. Cancer Immunol Res. 3:412. PubMed