Alexa Fluor® 647 anti-mouse H-2Kκ
Description
The 36-7-5 antibody reacts with the H-2Kκ MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kκ haplotype. H-2Kκ is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Goudeau B, et al. 2003. P. Natl. Acad. Sci. USA 100:15800.
2. Johnson RM. 2004. Infect. Immun. 72:3951.
3. Ardehali A, et al. 1995. Circulation 92:450.