Alexa Fluor® 488 anti-mouse CD49e

Antibodies Single
Sony
5H10-27(MFR5)
Immunofluorescence
Rat IgG2a, κ
Mouse
(C57BL/6 x A/J)F<SUB>1</SUB> mouse mast cell line
100 µg
1119050
$226.00

Description

CD49e is a 135 kD protein, also known as α5 integrin or VLA-5 α chain. It is a member of the integrin family, expressed on thymocytes, splenic B cells, activated T cells, and mast cells. CD49e associates with CD29 (integrin β1 chain) to form the fibronectin receptor (VLA-5). CD49e plays a critical role in both adhesion and T cell costimulation. The primary ligand for CD49e/CD29 (VLA-5) is fibronectin. The 5H10-27(MFR5) antibody has been shown to block CD49e mediated interactions and promote the in vitro stimulation of CD8+ T cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
2. Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
3. Rich S, et al. 1996. J. Immunol. 157:2916. (Block Costim))
4. Uhlenkott CE, et al. 1996. Clin. Exp. Metastasis 14:125. (Block)
5. Schultz JF, et al. 1995. J. Biol. Chem. 270:11522. (Block)
6. Yang JT, et al. 1996. Mol. Biol. Cell. 7:1737. (IHC)
7. Cuklerman E, et al. 2001. Science 294:1708. (IHC)
8. Jia W, et al. 2005. Blood 106:3854. (FC)