Description

CD195 is a 45 kD chemokine receptor also known as CCR5. CD195 is a seven transmembrane-spanning G protein-associated molecule expressed on macrophages, a T cell subset, and in the heart, liver, and spleen. CD195 regulates lymphocyte chemotaxis and transendothelial migration during inflammatory processes. CD195 interacts with several ligands including RANTES, MCP-1, MIP-1α, and MIP-1β.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 microg per 10⁶ cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Mao A, et al. 2005. J. Immunol. 175:5146. (FC) PubMed
2. Ishida Y, et al. 2007. Am J Pathol.170:843.(FC) PubMed
3. Zeiser Z, et al. 2008. Blood 111:453. (FC) PubMed
4. Sharma R, et al. 2009. J. Immunol.. 183:3212 (FC) PubMed
5. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed