7-AAD Viability Staining Solution

Buffers/Solutions
Sony
Flow Cytometry
2702020
$61.00

Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Formulation

PBS, pH 7.2 with 0.09% (w/v) sodium azide.

Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 microL of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

 

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