7-AAD Viability Staining Solution

Buffers/Solutions
Sony
Flow Cytometry
2702015

Description

7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).

Formulation

PBS, pH 7.2 with 0.09% (w/v) sodium azide.

Recommended Usage

For dead cell exclusion, resuspend cell pellet in 0.5 mL of Cell Staining Buffer and add 5 microL of 7-AAD per million cells and incubate for 5-10 minutes in the dark before analysis. The analysis of the cells should be done as soon as possible after the incubation with the dye.

 

References

1. Paterson AM, et al. 2011. J. Immunol. 187:1097. PubMed
2. Wingren M, et al. 2012. Arterioscler Thromb Vasc Biol. 32:2000. PubMed
3. Sandu SK, et al. 2012. PNAS 109:20047. PubMed
4. Evdokimova V, et al. 2012. Sci Signal. 5:92. PubMed
5. Choudhury KR, et al. 2012. PLoS One. 7:e51415. PubMed
6. Wu Y, et al. 2013. J. Immunol. 190:1859. PubMed
7. Harker KS, et al. 2013. J. Leukoc Biol. 93:789. PubMed
8. Zhang ZN, et al. 2013. Clin Chem. 59:1175. PubMed
9. Mikhak Z, et al. 2013. J Exp Med. 210:1855. PubMed
10. Cohen HB, et al. 2013. Blood. 122:1935. PubMed
11. Hufbauer M, et al. 2013. J. Virol. 87:12158. PubMed
12. Turner JE, et al. 2103. J Exp Med. PubMed
13. Teng T, et al. 2013. Mol Cell Biol. 33:4660. PubMed
14. Hussain MS, et al. 2013. Hum Mol Genet. 22:5199. PubMed
15. Turner JE, et al. 2013 J. Exp Med. 210:2951. PubMed
16. Kong W, et al. 2014. Cell Immunol. 287:1. PubMed
17. Fell LH, et al. 2014. Nephrol Dial Transplant. PubMed
18. Kraaji MD, et al. 2014. Cytokine. 67:7. PubMed
19. Schneider C, et al. 2014. PNAS. 111:8185. PubMed
20. Ermann J, et al. 2014. PNAS. 111:2559. PubMed
21. Cao L, et al. 2014. J Virol. 88:8656. PubMed
22. Ni PP, et al. 2014. J Immunol. 193:1778. PubMed
23. Wedel J, et al. 2014. J Leukoc Biol. 96:453. PubMed
24. Jaworska J, et al. 2015. J Immunol. 194:325. PubMed