Photomultiplier tubes (32-channel PMTs, single-channel PMTs, and single-channel IR-sensitive PMTs)
Fluorescence sensitivity (molecules of equivalent soluble fluorochrome)*
FITC ≤ 58 MESF; PE ≤ 4 MESF; APC ≤ 7 MESF
*Specifications listed are an average of data obtained from multiple systems.
Pulse parameter
Area, Height, Width (all channels)
Signal resolution
Height (20 bit), Area (32 bit)
Sampling frequency: 60 MHz
Detectable particle size
160 nm with SSC
Fluorescence resolution
CV < 3% for the singlet peak of propidium iodide-stained Chicken Erythrocyte Nuclei (CEN)
Event rate
40,000 events per second
Fluidics
Sample flow rate
~33 μL/min–200 μL/min
Cleaning modes
Priming, Probe Wash, Flow Cell Purge, Decontamination (with bleach), Hardware Shutdown (performed before system shutdown), Automatic Shutdown (programmed system shutdown after completion of Auto Acquisition)
System fluidics
10-L capacity Sheath Tank and 10-L Capacity Waste Tank Sheath fluid (distilled water or phosphate buffered saline)
System software
Software
ID7000 Acquisition and Analysis Software
Supported file types
exdat and FCS 3.1
AutoSampler
Sample loading options
Multiwell plates: 96-well plate: standard height flat/V/U bottom, 96-well half deep V bottom, 96-well deep U bottom, 384-well standard flat bottom
Tubes: 24-tube rack that accommodates 5-mL (12 x 75-mm) polystyrene/polypropylene tubes
Minimum sample volume*
5-mL tube: 100 µL in standard mode/50 µL in low-volume mode, 96-well plate: 55 µL in standard mode/10 µL in low-volume mode, 384-well plate: 40 µL in standard mode/10 µL in low-volume mode
*Minimum volumes provided for the sample vessel type are averages of data obtained from multiple experiments.
Carryover
< 0.1% (when measured with inner and outer Probe Wash)
Throughput
96-well plate in 19 minutes (Based on an acquisition time of 2 seconds per well, no agitation, and no probe wash. The value reported is an average of multiple experiments.)
Sample agitation
Multiple mixing modes: Once at the beginning of acquisition, Cyclic, or Continuous Mixing
Temperature control
From ambient temperature to 4°C with a Peltier sample cooling module
