Instrument Specifications

Optics Excitation lasers 488 nm, 638 nm, 405 nm, 561 nm
Output power Optical fiber output : 405 nm: (10 mW max.), 488 nm, 638 nm, and 561 nm: (36 mW max.)
Beam alignment Dual axis optical system
Detection parameters 12 fluorescence + 2 scatter
Pulse measurement Height, Area, Width
Fluidics Sample tube Single, auto-loading tube
Tube types 0.5-mL, 1.5-mL, 5-mL and 15-mL tubes
Sort Devices 2-way tube, 4-way tube, multiwell plates, PCR plates
Temperature control 5°C, 37°C (electric cooling method)
Agitation unit Eccentric rotation
Magnetic drive 300 rpm speed
Sorting chip size 70 μm, 100 μm
Sort Performance Event rate 70,000 eps
Sorting speed

Automated frequency search range

  • 70 μm : 40 kHz to 52 kHz
  • 100 μm [Targeted] setting : 21 kHz to 23.5 kHz
  • 100 μm [Standard] setting : 27 kHz to 31 kHz
  • 130 μm : 10 kHz to 12 kHz

Using the 70-μm sorting chip at 52 kHz and a threshold rate of 12,000 events per second,
purity >98% and recovery >80% can be achieved. The yield obtained is based on Poisson’s statistics. Higher threshold events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics.
Scatter resolution 0.5 µm
Fluorescence resolution <3.0% coefficient of variation (CV): PI stained CEN
Fluorescence sensitivity FITC ≤95 MESF, PE ≤90 MESF
Ancillary Dimensions W: 21.7” (55 cm) x D: 21.7” (55 cm) x H: 28.4” (72 cm)
Fluidics cart W: 33.9” (86 cm) x D: 17.3” (44 cm) x H: 11.8” (30 cm)
Weight 231 lb (105 kg)
Fluidics cart 108 lb (49 kg) (dry weight)
LCD panel 7-inch, 800 x 480 pixels
Power supply 100–240 V, 50/60 Hz
Power consumption 600 W (max.)
Operating temperature 19.5°C to 27.5°C
Relative humidity 20% to 80%
Compliance Operating system Microsoft® Windows® 10 Professional, 64 bit
Data file structure Flow Cytometry Standard (FCS) 3.0 or 3.1
Safety Standards Compliance UL, CE, CSA

Filter Guide

ma900_filter_guide