SP6800 System Analyzer

The SP6800 spectral flow analyzer improves sensitivity and simplifies application design, workflow, and analysis over conventional flow cytometers. This is achieved using spectral analysis technology, advanced electronics, and patented optical technologies. These capabilities, unique to Sony Biotechnology systems, allow experienced and novice flow cytometrists to achieve greater flexibility for panel design and more accurate visualization of results.

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Software

The SP6800 software is easy to learn and use. It guides researchers from set-up to panel design, acquisition, analysis, and shutdown.

User preferences allow users and administrators set up options for overall instrument operation and experiment set up to facilitate use and unattended operation procedures.

System Start Up

At start up Align Check and Performance QC wizards check instrument calibrations, using beads to ensure the instrument is operating optimally. On screen instructions guide the user through procedures, then display progress and report results. The performance report displays MESF, Q, and B values to describe real-time fluorescence detection performance. If desired, Align Check and Performance QC reports can be displayed in historical context.

Standardization

Standardization mode sets the system to a master specification to eliminate variability in a single instrument or among instruments located across sites. This unique capability allows experiments performed on any Sony cell analyzer to produce highly reliable, accurate, and reproducible results. This function also eliminates setup subjectivity for collaborative or long term studies with different operators or experience levels across sites.

When engaged, standardization mode sets the SP6800 to predetermined global settings that eliminate instrument variability.

Spectral Library

The Spectral Library lets users create a personal reagent library that simplifies experiment creation and saves time. An Acquisition Wizard assists the users with step by step instructions to acquire and analyze single positive controls for your spectral library. Once acquired the spectral reference for that reagent including the spectral index are available for future use. Information from the spectral library is available to users with a simple click improving accuracy and streamline panel design.

Experiment Creation

Experiments can be created using a template, an existing experiment, a single stained, or multicolor assay, in the Create Experiment window. Users can point and click to choose (or edit) an existing experiment and can easily select templates for wells or plates when creating a new experiment. A setup Assay Wizard guides users through the creation of a singlestained or multicolor assay simplifying experiment creation.

Acquisition Functions and Analysis

All acquisition functions, including instrument settings are controlled from the Acquisition Window. Worksheet tools let users choose how the data is displayed- (such as plot types), and customize for their analysis needs. Plots and statistics provide real-time information during acquisition. To increase sample acquisition to the cuvette, a variable booster lets users set acquisition speed from low (33ul/min) normal or high (250ul/min) offering flexibility.

System Shutdown

On shutdown, the SP6800 software guides the user though pre –shutdown cleaning and shuts down the instrument automatically. Software wizards are also available to guide users through Bleach Cleaning and Rinse procedures.

FCS Express from De Novo Software

Spectral Analyzers from Sony include FCS Express, from De Novo Software.
FCS Express offers a range of new analysis tools from live gating to batch
analysis. Native support for Sony Spectral data files to enable sophisticated
data transformations and visualizations such as spectral overlays, tSNE,
Spade, and heat maps.

Live Gating

Dot plots and spectral plots can be set up with live gating. Live gating allows users to find positive and negative populations quickly and easily for added assurance about target populations.

Flexible Analysis

Flexible data analysis can include integrated spreadsheets, custom calculations, charting and regression analysis.

Batch and Report

Batch analysis lets you process any number of samples with one click. To support presentation, data can be exported directly to PowerPoint, PDF, and Excel.