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- Sony Biotechnology
- Sony Biotechnology
Recent advances in genome editing and the application of fluorescent proteins have accelerated Interest in isolating specific populations of brain cells from mixed populations. Researchers are also using cell sorting to isolate single cells for expansion and analysis.
Follow these 5 guidelines to address the challenge of obtaining single cell suspensions from delicate brain tissues.
Several studies have been published in which scientists have successfully isolated stem cells from whole brain homogenate using cell sorting. The references below provide more detailed information about preparing and sorting brain tissue.
Daynac, Mathieu, et al. "Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics." J Vis Exp. (103 (2015). PubMed
Wylot, Bartosz, et al. "Isolation of vascular endothelial cells from intact and injured murine brain cortex—technical issues and pitfalls in FACS analysis of the nervous tissue." Cytometry A. 10 (2015): 908-920. PubMed
Guez-Barber, Danielle, et al. "FACS purification of immunolabeled cell types from adult rat brain." J Neurosci Methods 1 (2012): 10-18. PubMed