The ID7000™ Spectral Cell Analyzer from Sony Biotechnology has set a new standard in high-parameter immunophenotyping, providing researchers with the ability to explore cellular populations with exceptional depth and flexibility. Its advanced technology facilitates deep analysis, unlocking insights into the intricate workings of the immune system. Consistency in data and among users over time is paramount in scientific research, for which the integrity of results must be beyond reproach. Even with sophisticated instruments like the ID7000, variability can arise from numerous sources, including sample preparation and instrument calibration, which can compromise data quality.

Sony’s Standardization mode addresses these issues by ensuring that key instrument parameters are precisely calibrated, allowing for consistent, reliable results that researchers can trust. This feature, while not new, remains a cornerstone of the ID7000’s design philosophy, reflecting Sony’s commitment to excellence in reproducibility.

This presentation will demonstrate how researchers at IMU Biosciences, where acquisitions might be completed over the course of days, weeks, and years, have applied Standardization mode, resulting in increased temporal precision in high-dimensional datasets.

Additionally, we will discuss practical strategies for implementing this mode effectively in everyday experimental workflows, including best practices for calibration, panel design, and data analysis. By leveraging the full potential of Standardization mode, users can enhance the reliability of their immunophenotyping studies, driving more accurate and impactful results in both research and clinical applications.

Key Learning Objectives:

  • Understand the difference between Normal mode and Standardization mode
  • Understand the different sources of variability in longitudinal studies
  • Review data that demonstrates the utility of Standardization mode
  • Understand the key benefits of Standardization mode
  • Learn the different applications that Standardization mode can be applied to

 

Who should attend

This webinar will cover the benefits and use of Standardization mode on the ID7000 Spectral Cell Analyzer as experienced by scientists at IMU Biosciences. The content will be of interest to those who want to see how inter- and intra-instrument studies can be executed without compromising data quality or introducing variability. Attendees will learn about best practices for calibration, panel design, and data analysis within Standardization mode.

 

Speakers

Portrait of Paul Rause, PhDPaul Rouse, PhD
Platform R&D Scientist
Expert in Thymus Biology and Spectral Flow Cytometry
IMU Biosciences

Paul is a passionate and experienced Platform R&D Scientist at IMU Biosciences with over eight years of research experience spanning both industry and academia. Specializing in thymus biology and T-cell development, Paul has developed deep expertise in using spectral flow cytometry to characterize immune lineages.

After earning a PhD focused on reconstituting the T-cell repertoire through direct lineage reprogramming strategies for thymic epithelial cells, Paul further advanced this field in his postdoc, where he pioneered a novel method for organotypic thymic co-cultures. This work not only deepened Paul's knowledge of immunology and T-cell biology but also refined his technical acumen in spectral flow cytometry—the gold standard for characterizing T-cell identity.

Paul is currently employed by IMU Biosciences as a Platform R&D Scientist, aiming to utilize the ID7000 to generate deep immunophenotyping datasets for predictive modelling and precision medicine.