Sony CGX10 Cell Isolation System: An Introduction and Examples of Use in Protocols for Generating Cell Therapy Products Using Multiparametric Selection
Presented by Select Science
Commonly employed cell purification platforms for large-scale enrichment and isolation of immune cells or stem cells for cell therapy applications, separate cells based on a single surface antigen. Enrichment based on multiple cell surface markers or different expression levels of these markers often requires the use of a traditional cell sorter.
Here we describe the use of the Sony CGX10 Cell Isolation System, a fully closed system suitable for GMP-compliant cell sorting. The CGX10 Cell Isolation System utilizes a novel microfluidics technology to achieve high purity cell isolation within a closed and sterile microfluidics chip. We will also discuss some examples and share data on utilizing the CGX10 Cell Isolation System in different cell and gene therapy manufacturing workflows. The system enables fully closed, high speed, high purity, multiparametric, fluorescence-based selection of target cells.
Key Learning objectives:
- Utilization of multiparametric selection in cell and gene therapy manufacturing workflows
- Deployment of GMP-ready fluorescence-based cell sorting in a typical CAR-T workflows
- Advantages of using the CGX10 Cell Isolation System in cell and gene therapy manufacturing workflows
Speakers
Dr. Manoja Eswara
Senior Development Manager
Centre for Commercialization of Regenerative Medicine
Manoja Eswara is a Senior Development Manager at CCRM in Toronto. At CCRM, Manoja is engaged in management and execution of contract services for academic and industrial clients, specializing in the areas of gene editing, iPSC reprogramming, differentiation and scale up and Immunotherapy. She is also engaged in technology evaluation, translation, process development and tech transfer to GMP, in the fields of cell and gene therapy. Prior to CCRM, Dr. Eswara did her postdoctoral fellowship at Lunenfeld-Tanenbaum Research Institute, Toronto, studying the role of histone chaperones in cancer epigenetics. Dr. Eswara received her PhD in molecular biology from the University of Guelph.
Dr. Aditi Singh
Global Senior Product Manager
Sony Biotechnology Inc.
Aditi is currently working as Global Senior Product Manager at Sony Biotechnology leading the launch strategy and management of instrument utilized in cell and gene therapy manufacturing workflow. Her goal through this role is to improve patient care by making personalized medicine (cell therapy) more accessible to patients. Aditi has a Ph.D degree in Cell and Molecular Biology from University of Heidelberg, Germany and post-doctoral experience from the University of California, San Diego where she worked in the fields of infectious diseases and mRNA biology.
