Screening for new therapeutically effective T-cell receptors (TCRs) using cell sorting, nanovial technology, and single-cell RNA sequencing
Presented by Select Science
In recent years, cell-based therapies have been tremendously successful in treating hematologic malignancies. Efforts have continued to expand treatment to other chronic diseases. However, the underlying genetic state and therapeutic functions of these cells, such as secretion of cytokines, are still not well understood. New approaches to analyze and sort live, intact cells based on combinations of surface markers and secretions can accelerate the development of cell therapies by isolating therapeutic immune cells with highly tuned functional responses for downstream functional, transcriptomic, or genomic analyses.
Join this webinar to hear how Dr. Dino Di Carlo’s team is using the Sony SH800 cell sorter to obtain purified subsets of chemically modified cavity-containing hydrogel microparticles, called nanovials. These microparticles are then used in a multiomic and multiparametric approach to discover the most therapeutically effective TCRs while simultaneously deconvolving their cognate binding peptides using single-cell sequencing.
Key Learning objectives:
- Learn how cell sorting, nanovial technology, and single-cell RNA sequencing can be used together to screen and discover new TCRs or chimeric antigen receptors (CARs).
- Find out how microfluidics chip based sorting of nanovials enables enrichment of T cells with even low-affinity TCRs based on avidity-based binding that outperforms tetramer staining.
- Uncover tools for using combinations of fluorescence area and height gates to sort high-secreting cells using the Sony SH800 cell sorter.
Who should attend
The webinar will highlight new tools that are valuable to immunology researchers studying antigen-specific T cells and their functions, cell engineers looking to find the best genetic modifications for cellular immunotherapies, and researchers generally interested in how secretions drive cell function.Participants will learn of improved approaches to sort these microparticles using a microfluidics chip based sorter and perform multiomic analyses to uncover rare TCRs and their cognate peptide-MHCs.
Speaker
Dino Di Carlo, PhD
Professor in Engineering and Medicine, UCLA
Dr. Di Carlo is a professor, inventor, and entrepreneur with more than 170 publications and 60 issued patents. He is the Armond and Elena Hairapetian Endowed Professor in Engineering and Medicine at the University of California Los Angeles (UCLA). His academic research has focused on the interface of micro and nanosystems, data science, and the ultimate limits of biology (single cells and molecules).
Felis Koo, MS
Doctoral Candidate, UCLA
Felis Koo, MS, is a Doctoral Candidate at UCLA. Her research is pioneering the use of hydrogel nanovials for advanced single-cell multiomic analyses. She was awarded the Biomicrofluidics Best Paper Award for her work selecting single mesenchymal stem cells on nanovials based on extracellular vesicle (EV) secretion.
Please note neither UCLA nor The Regents of the University of California (The UC Regents) endorses any product, service or activity related to the Sony webinar hosted by Select Science and nothing in these materials will be construed to imply that UCLA or The UC Regents authorizes, supports, endorses, or sponsors any product or service resulting from the Sony webinar hosted by Select Science.