Identification of Frequent Autoreactive B-cell Response in Pancreatic Ductal Adenocarcinoma Using Cell Sorting and Single-Cell Sequencing
B-cell responses within pancreatic ductal adenocarcinoma (PDAC) tumors are associated with improved patient outcomes, yet the specific triggering antigens remain largely unknown. In this webinar, Dr. Min Yao will present a methodology that combines cell sorting, single-cell RNA sequencing (scRNA-Seq), and immunoglobulin (Ig) sequencing of tumor-infiltrating immune cells to uncover these antigens. Dr. Yao's study shows evidence of various immune responses, including activated T and B cells, germinal center reactions, and plasma cells producing antibodies targeting intracellular self-antigens in pancreatic cancer tissue. These findings indicate a potential link between chronic inflammation in pancreatic cancer and an enhanced adaptive immune response, which could have implications for treatment development.
Key Learning Objectives:
- Understand the immune micro-environment in PDAC using the SH800 Cell Sorter and 10x Genomics single-cell analysis tools
- Learn how to identify specific antigens associated with B-cell responses in clinical samples
- Gain insights on autoreactive B-cell responses in PDAC and their potential clinical impact
Who should attend
Researchers, medical professionals, and pharmaceutical industry experts interested in cancer immunology and oncology, particularly those focusing on pancreatic cancer diagnosis, treatment, and the development of immunotherapy and targeted therapy strategies.
Speakers
Min Yao, PhD
Senior Research Scientist
Sanders Tri-Institutional Therapeutics Discovery Institute
Min Yao, PhD, is a senior research scientist at the Sanders Tri-Institutional Therapeutics Discovery Institute, a collaborative effort between Memorial Sloan Kettering Cancer Center, The Rockefeller University, and Weill Cornell Medicine in New York. Dr. Yao specializes in antibody development and discovery, leveraging advanced techniques such as antigen-specific B cell sorting and single-cell sequencing. Previously, he conducted postdoctoral research at Cold Spring Harbor Laboratory, focusing on studying B cell response in pancreatic cancer under the mentorship of Dr. Douglas Fearon. Dr. Yao earned his doctoral degree from the University of Kansas Medical Center, where he investigated breast cancer progression under the guidance of Dr. Nikki Cheng.
Patrick Murphy, PhD
Senior Science & Technology Advisor
10x Genomics
Patrick is a Senior Science and Technology Advisor for 10x Genomics. He completed his PhD in Immunology at the University of Rochester, where he studied the impact of apoptotic cell clearance in macrophage inflammatory responses. Following that, he worked at the University of Pittsburgh as a research assistant professor studying tumor infiltrating lymphocytes (TIL) as a treatment for metastatic uveal melanoma. During his time at Pitt, Patrick made extensive use of single-cell technologies. Subsequently he joined 10x Genomics, where he supports these technologies in his current position.