High-Dimensional High-Throughput Rare Event Immunophenotyping on the ID7000™ Spectral Cell Analyzer
Presented by FluoroFinder
The ID7000™ spectral cell analyzer is ideal for high-dimensional flow cytometry. More lasers and many more detectors mean more fluorochromes can be resolved in combination. At 42 colors, the marker resolution is sufficient to support either directed hierarchical gating or undirected clustering algorithms. In addition, the ID7000 AutoSampler provides high-throughput analysis with better sample integrity, less sample waste, essentially zero carryover, and walk-away automation. These high-dimensional high-throughput capabilities combine to make the ID7000 exceptional for rare event screening. Populations smaller than 0.1% of total events were discerned with clear agreement between the results obtained by hierarchical gating and the clustering algorithm.
Learning objectives:
- Understand how thoughtful marker assignment can minimize the impact of spillover spreading on marker resolution in high-parameter panels.
- Learn how truly walk-away automated sampling with essentially zero carryover can be achieved using the various features of the ID7000 AutoSampler.
- See how high-parameter data can be analyzed by either traditional plotting and gating or by an undirected clustering algorithm to obtain the same results, even on rare populations
Who should attend
This webinar will provide insights for researchers who want to learn how spectral technology, such as that on the ID7000, can increase flexibility when using greater than 20 markers in multicolor flow cytometry experiments.
Speaker
Jeff Clapper
Senior Field Applications Scientist
Sony Biotechnology Inc.
Jeff Clapper has been serving the flow cytometry community for more than 35 years in various roles from academic research to instrument sales and marketing. He is currently performing a dual role as the sales representative and field applications scientist for Sony Biotechnology Inc. in the Maryland area.
