Ensuring Consistent Results in Spectral Flow Cytometry – Standardized inter- and intra-platform data using the ID7000™ and FP7000 spectral systems
Maintaining consistent performance between spectral flow cytometry experiments is crucial, particularly when acquiring single-stained controls and multicolor samples. Consistency allows for the reuse of spectral references in unmixing calculations. Uniform performance becomes even more important when multiple instruments are employed for data collection in cross-site studies. Flow cytometers from Sony Biotechnology offer a standardization feature for both the ID7000 Spectral Cell Analyzers and the new FP7000 Spectral Cell Sorters. This capability facilitates standardization across different platforms and enables easier panel portability.
In this webinar, we will share the highlights of a broad study describing how bead and cell data was collected using various instruments at different PMT voltage settings and at multiple time points to evaluate performance over time, both within and across platforms. The results demonstrate that equivalent signals were achieved across the different test scenarios. Standardization effectively controlled for variations within each instrument over time, and among multiple instruments, while allowing for optimal performance.
This feature enables users to increase workflow efficiency through reuse of spectral references at varying voltage settings, conserving resources and bolstering confidence in data quality.
Key Learning Objectives:
- Explore the standardization feature for the ID7000 and FP7000 systems
- From study highlights, learn how standardization controls variation over time and across platforms
- Get tips to streamline workflows by reusing spectral references
Who should attend
This webinar is ideal for researchers looking to use the same panel across multiple instruments, users managing data collection across different sites, and QA specialists or engineers focused on optimizing and maintaining data quality in spectral flow cytometry workflows.
Speakers
Koji Futamura
Senior Manager, Global Scientific Affairs
Sony Biotechnology Inc.
Since joining Sony Corporation in 2009, Koji Futamura has been involved in the flow cytometer business. After earning his master’s degree from the University of Tokyo, he gained experience through collaborative research with leading institutions and a research fellowship at the Wyss Institute at Harvard University. He led the development and commercialization of the flagship ID7000 Spectral Cell Analyzer. He received the Sony Outstanding Engineer Award in 2020 and the Kenjiro Sakurai Memorial Award in 2022 and is now engaged in global product planning and R&D collaboration.
Olivia Bracken, PhD
Post Doctoral Researcher
University College London
Olivia Bracken is a post-doc in Professor Arne Akbar’s lab at University College London. Her research focuses on elucidating the mechanisms of cellular cytotoxicity in the aging immune system. She conducted her PhD studies in Professor Derek Gilroy’s lab at UCL, focusing on the role of inflammatory monocytes in the resolution of inflammation. Olivia is a specialist in spectral flow cytometry and is excited by the opportunities this technology brings.