Enhanced Single-Cell DNA Methylation Analysis Using Highly Parallelized Barcoding Coupled with Nuclei Sorting
The epigenetic landscape of the human brain undergoes transformations during development, when DNA methylation plays a crucial role. While traditional bulk techniques lack the precision of single-cell approaches, the latter have been constrained by high costs and labor intensity. ScaleBio's innovative combinatorial indexing technology, coupled with the SH800 Cell Sorter, revolutionizes single-cell DNA methylation analysis with a cost-effective and efficient plate-based workflow, providing high-throughput capabilities. This approach ensures high recovery rates, robust cytosine coverage, and the identification of cell-type-specific clusters during various developmental stages. Compared to bulk profiles, it reveals distinct single-cell methylation patterns, offering unparalleled insights into developmental methylation alterations and cellular heterogeneity.
Key Learning Objectives:
- Explore this combinatorial indexing technology for precise and efficient single-cell DNA methylation analysis.
- Learn how the SH800 Cell Sorter improves the outcomes of single-cell methylation studies.
- Discover insights into developmental methylation alterations and cellular heterogeneity.
Who should attend
This webinar is tailored for epigenetics researchers specializing in DNA methylation dynamics during brain development, as well as biotech and genomics professionals, and lab technicians interested in advanced DNA methylation site identification and optimizing their workflows for single-cell DNA methylation analysis.
Speaker
Sanika Khare, PhD
Application Scientist
Scale Biosciences
Dr. Sanika Khare is an application scientist with Scale Biosciences, a life sciences company focused on innovative single-cell library preparation approaches. At Scale, Dr. Khare is working on developing cost-effective single-cell profiling techniques with applications across the fields of genomics and epigenomics to enable biological discovery.
Dr. Khare received a Masters and PhD from the University of Pennsylvania with research focusing on metabolism and oncology.