An integrated multimodal analysis workflow to sort, sequence, and characterize antigen-specific B or T cells
Presented by Select Science
B and T cells perform a vital function in the adaptive immune system. They recognize molecules the body has determined are foreign, called antigens, and subsequently trigger events that will clear those molecules and the pathogens they are derived from, such as viruses or bacteria. However, isolating antigen-specific B and T cells out of a complex sample with traditional techniques has proved challenging and time consuming for those in drug development. To solve this problem, we developed a workflow to assemble antigens or peptides of interest with unique barcodes and fluorescent reporters, which can then be used for cell sorting.
This webinar discusses the use of Barcode Enabled Antigen Mapping (BEAM), a multiplexed antigen screening workflow for empowering the rapid discovery of antigen-specific B-cell (BEAM-Ab) and T-cell (BEAM-T) clonotypes. The technology is built on the proven Chromium® Single Cell Immune Profiling solution. The unique workflow allows researchers to obtain comprehensive antigen-specific cellular profiles, including full-length paired V(D)J sequences, gene expression, and cell surface proteins, from the same single cell to accelerate therapeutic discovery.
In this webinar you will learn how to:
- Sort and characterize fluorescently labeled antigen-specific B or T cells with a customized gating strategy to enrich for binders and exclude non-binding populations
- Generate tens to hundreds of high-quality antigen-specific hits from a single sample in one week
- Capture unmatched profiles of antigen-specific B and T cells, including full-length, paired V(D)J sequences, gene expression, and surface protein expression, at single-cell resolution
- Discover rare and/or therapeutically relevant antigen-specific clonotypes to advance the next immunotherapeutic breakthrough
Who should attend
This webinar will provide insights for researchers who want to learn new approaches for successfully profiling antigen-specific T and B cells for high-throughput single-cell sequencing assays.
Speaker
Patrick Murphy, PhD
Science and Technology Advisor
10x Genomics
Patrick is a Science and Technology Advisor for 10x Genomics. He completed his PhD in Immunology at the University of Rochester, where he studied the impact of apoptotic cell clearance in macrophage inflammatory responses. Following that, he worked at the University of Pittsburgh as a research assistant professor studying tumor infiltrating lymphocytes (TIL) as a treatment for metastatic uveal melanoma. During his time at Pitt, Patrick made extensive use of single-cell technologies. Subsequently he joined 10x Genomics, where he supports these technologies in his current position.