Advancing Whole-Blood Immunophenotyping for Cross-Species Immune Cell Mapping Using the ID7000™ Spectral Cell Analyzer
Join this webinar to learn how experts from VIB Flow Core successfully transitioned from conventional to spectral flow cytometry using the ID7000™ Spectral Cell Analyzer. Using a 22-color human whole-blood immunophenotyping panel, the team leveraged the ID7000's full-spectrum detection and autofluorescence extraction capabilities to clearly resolve eosinophils and accurately quantify FcγRIIA and FcRn - all without modifying their existing panel design. This panel formed the basis of the FcγR expression atlas published by Van Damme et al. in Science Immunology (January 2026), highlighting its significance for advancing FcγR biology research.
The presenters will also demonstrate how the ID7000's advanced workflow capabilities enabled comprehensive mapping of complex immune cell populations across species. Learn how features such as AF Finder identify highly autofluorescent cell populations, how autofluorescence signatures can be incorporated into spectral unmixing to resolve challenging populations while preserving panel integrity, and how advanced spectral unmixing tools help manage donor-specific autofluorescence and optimize spectral reference controls for reproducible, high-quality data.
Learning Objectives:
- Learn how the ID7000™ Spectral Cell Analyzer simplifies migration of immune panels from conventional flow cytometers and supports successful implementation of spectral flow cytometry workflows.
- Gain practical strategies for spectral unmixing, autofluorescence management, creating high-quality spectral reference controls, and leveraging the spectral reference library to optimize panel performance.
Who should attend
This webinar is ideal for researchers and managers in large labs and core facilities who are considering transitioning to spectral flow cytometry. It will be of special interest to scientists who require reliable detection capabilities for studying the expression of Fc gamma receptors (FcγRs), the neonatal Fc receptor (FcRn), and other regulators.
Speakers
Gert Van Isterdael
Core Head, VIB Flow Core Ghent
As head of the VIB Flow Core, Gert provides flow cytometry expertise to the users of the core facility by giving technical help to the scientists, maintaining the instruments, implementing high quality SOPs, and providing both experimental setup and data analysis consultations. He has contributed to the Flow Core’s development of novel automated analysis techniques for multiparameter flow cytometry data to remove user bias, in close collaboration with the on-site Bioinformatics team.
Katrien Van der Borght
Flow Cytometry Expert, VIB Flow Core Ghent
Katrien obtained her master’s degree in Biomedical Sciences in 2009. She completed her PhD in Immunology in 2017 in the lab of Bart Lambrecht (VIB-UGent), where she continued as a postdoctoral researcher for five years. Her research focused on the mouse immune system in the context of heart inflammation and lung allergy. Since May 2023, she has been part of the VIB Flow Core, where she supports researchers in designing and performing high quality flow cytometry experiments.