Sony Biotechnology offers over 9,000 high quality monoclonal antibody products to support your research. These include widely published clones conjugated to bright dyes such as Brilliant Violet, and PE/Dazzle.
Browse the technical tools section for practical advice on fluorochromes, panel design, as protocols for surface and intracellular (cytokine, transcription factor, and phosphoprotein) staining. The Biology section maps markers and reagents appropriate to T-cell, B-cell, Cancer and Stem Cell investigations. This is where you’ll also find sample panels.
Fluorochromes
Sony Biotechnologies is committed to advancing science through innovations in both flow cytometry instrumentation and reagents. At Sony we are committed to increasing the number and quality of fluorochromes available to continue to improve your results. Please visit our website and speak with your sales representative to learn about our latest releases.
Over the last several years the number of fluorochromes available for flow cytometry has dramatically increased. These next few pages will provide basic information about our current fluorochrome selection.
When selecting fluorochromes it is important to know the laser and filter configuration of your instrument. Spectral overlap is also a consideration. With compensation one can separate the overlap however some signal will be lost. This is especially true with a conventional flow cytometer compared to a spectral analyzer(more about this in panel design).
Fluorochromes for Blue Laser (488 nm)
The Blue (488nm) Laser is the most common laser for flow cytometry research. Blue lasers are generally equipped to detect 2-5 flurochromes.
Emission Spectra of Fluorochromes excited by the Blue Laser
Alexa Fluor® 488
| Relative brightness | +++ |
| Recommended filter | 530/30 |
| Excitation max (nm) | 495 |
| Emission max (nm) | 519 |
| Recommended for | Medium to high density markers; intracellular markers |
| Incompatible dyes | FITC, BD Horizon Brilliant™Blue 515 |
| Comments |
FITC
| Relative brightness | +++ |
| Recommended filter | 530/30 |
| Excitation max (nm) | 493 |
| Emission max (nm) | 525 |
| Recommended for | Medium to high density markers |
| Incompatible dyes | Alexa Fluor®488, BD Horizon Brilliant™Blue 515 |
| Comments |
Pe
PE
| Relative brightness | +++++ |
| Recommended filter | 575/26 |
| Excitation max (nm) | 565 |
| Emission max (nm) | 575 |
| Recommended for | Low density markers |
| Incompatible dyes | |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers |
PE/Dazzle™ 594
| Relative brightness | +++++ |
| Recommended filter | 575/26 |
| Excitation max (nm) | 565 |
| Emission max (nm) | 575 |
| Recommended for | Low density markers |
| Incompatible dyes | PE-CF594 |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers |
PE-Cy™ 5
| Relative brightness | +++++ |
| Recommended filter | 670/14 |
| Excitation max (nm) | 565 |
| Emission max (nm) | 670 |
| Recommended for | Low density markers |
| Incompatible dyes | PerCP, PerCP-Cy™5.5 |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers, Tandem dye |
PerCP
| Relative brightness | +++ |
| Recommended filter | 695/40 |
| Excitation max (nm) | 482 |
| Emission max (nm) | 675 |
| Recommended for | Medium to high density markers |
| Incompatible dyes | PE-Cy5, PerCP-Cy5.5 |
| Comments |
PerCP-Cy 5.5
| Relative brightness | ++ |
| Recommended filter | 695/40 |
| Excitation max (nm) | 482 |
| Emission max (nm) | 690 |
| Recommended for | Medium to high density markers |
| Incompatible dyes | PerCP, PE-Cy5 |
| Comments | Tandem dye |
PE-Cy™7
| Relative brightness | ++++ |
| Recommended filter | 780/60 |
| Excitation max (nm) | 496 |
| Emission max (nm) | 785 |
| Recommended for | Low density markers |
| Incompatible dyes | APC-Cy™7 (on instruments with collinear lasers) |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. Protect from light and avoid prolonged exposure to paraformaldehyde containing fixatives. |
Fluorochromes for the Red Laser (638 nm)
Red lasers are another popular option for flow cytometry. Flow cytometers for the red laser can typically detect 1-3 fluorochromes.
Emission Spectra of Fluorochromes excited by the Red Laser
Fluorochromes for Violet Laser (405 nm)
APC
| Relative brightness | ++++ |
| Recommended filter | 660/20 |
| Excitation max (nm) | 650 |
| Emission max (nm) | 660 |
| Recommended for | Medium/Low density markers |
| Incompatible dyes | Alexa Fluor®647, eFluor®660 |
| Comments |
Alexa Fluor®647
Alexa Fluor®700
| Relative brightness | ++ |
| Recommended filter | 730/45 |
| Excitation max (nm) | 696 |
| Emission max (nm) | 679 |
| Recommended for | High density markers |
| Incompatible dyes | BD Horizon™ APC-R700 |
| Comments |
APC-Cy™7
| Relative brightness | + |
| Recommended filter | 780/60 |
| Excitation max (nm) | 650 |
| Emission max (nm) | 785 |
| Recommended for | High density markers |
| Incompatible dyes | APC-H7, PE-Cy™7 (on instruments with collinear lasers) |
| Comments | Tandem is sensitive to degradation. Protect from light and avoid prolonged exposure to paraformaldehyde containing fixatives |
Fluorochromes for Violet Laser (405 nm)
Violet lasers have increased in popularity in recent years due to the availability of many new bright dyes including the Brilliant Violet™ family. Most violet lasers can detect 2-6 fluorochromes.
When updating panels replacing dimmer fluorochromes such as Pacific Blue with very bright dyes such as Brilliant Violet™ 421, one may want to consider the antigen density of the markers. For example if Pacific Blue is used it is likely on a high antigen density (bright) marker such as CD4. You may want to put a lower antigen density or dimmer marker on the Brilliant Violet BV421 and put CD4 on a dimmer fluorochrome.
Brilliant Violet™421
| Relative brightness | +++++ |
| Recommended filter | 450/50 |
| Excitation max (nm) | 407 |
| Emission max (nm) | 421 |
| Recommended for | Low density markers |
| Incompatible dyes | Pacific Blue™, BD Horizon™ V450, eFluor®450, Violet Fluor™450 |
| Comments | Brilliant Violet 421 is currently the brightest marker for flow cytometry |
Pacific Blue™
| Relative brightness | + |
| Recommended filter | 450/50 |
| Excitation max (nm) | 405 |
| Emission max (nm) | 455 |
| Recommended for | High density markers |
| Incompatible dyes | Brilliant Violet™ 421, BD Horizon™ V450, eFluor®450, Violet Fluor™450 |
| Comments |
Brilliant Violet™ 510
| Relative brightness | +++ |
| Recommended filter | 525/50 |
| Excitation max (nm) | 405 |
| Emission max (nm) | 510 |
| Recommended for | Medium/high density markers |
| Incompatible dyes | BD Horizon™ V500, Pacific Orange™, LIVE/ DEAD® Fixable Aqua Dead Cell Stain |
| Comments |
Brilliant Violet™ 570
| Relative brightness | +++ |
| Recommended filter | 585/42 |
| Excitation max (nm) | 405 |
| Emission max (nm) | 570 |
| Recommended for | Medium density markers |
| Incompatible dyes | Pacific Orange™, Qdot® 565, Qdot® 585, eFluor® 565NC, eFluor® 585NC |
| Comments | May spillover into the PE channel |
Brilliant Violet™ 605
| Relative brightness | ++++ |
| Recommended filter | 610/20 |
| Excitation max (nm) | 405 |
| Emission max (nm) | 602 |
| Recommended for | Low density markers |
| Incompatible dyes | Qdot® 605, eFluor® 605NC |
| Comments |
Brilliant Violet™ 650
| Relative brightness | +++ |
| Recommended filter | 660/20 |
| Excitation max (nm) | 405 |
| Emission max (nm) | 645 |
| Recommended for | Medium density markers |
| Incompatible dyes | Qdot® 655, eFluor® 650NC |
| Comments | May spillover into APC and Alexa Fluor® 700 |
Brilliant Violet™ 711
| Relative brightness | +++ |
| Recommended filter | 710/50 |
| Excitation max (nm) | 407 |
| Emission max (nm) | 711 |
| Recommended for | Medium density markers |
| Incompatible dyes | Qdot® 705 and eFluor® 700NC |
| Comments |
Brilliant Violet™ 785
| Relative brightness | +++ |
| Recommended filter | 780/60 |
| Excitation max (nm) | 407 |
| Emission max (nm) | 785 |
| Recommended for | Medium density markers |
| Incompatible dyes | Qdot® 800 |
| Comments | Possible moderate spillover into the Alexa Fluor® 700 and PerCP-Cy™5.5 |
Green (532 nm) and Yellow Green (561 nm) Lasers
The green and yellow green lasers are typically used to detect PE and PE tandems. These dyes are typically brighter off the green/yellow green lasers compared to the traditional blue (488 nm) laser. Use of the green/yellow green laser can facilitate the spread of markers across lasers decreasing compensation requirements.
PE
| Relative brightness | +++++ |
| Recommended filter | 575/26 |
| Excitation max (nm) | 565 |
| Emission max (nm) | 575 |
| Recommended for | Low density markers |
| Incompatible dyes | |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers |
PE/Dazzle™ 594
| Relative brightness | +++++ |
| Recommended filter | 565/26 |
| Excitation max (nm) | 565 |
| Emission max (nm) | 575 |
| Recommended for | Low density markers |
| Incompatible dyes | PE-CF594 |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers |
PE-Cy™5
| Relative brightness | +++++ |
| Recommended filter | 670/14 |
| Excitation max (nm) | 565 |
| Emission max (nm) | 670 |
| Recommended for | Low density markers |
| Incompatible dyes | PerCP, PerCP-Cy™5.5 |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers, Tandem dye |
PE-Cy™7
| Relative brightness | ++++ |
| Recommended filter | 780/60 |
| Excitation max (nm) | 496 |
| Emission max (nm) | 785 |
| Recommended for | Low density markers |
| Incompatible dyes | APC-Cy™7 (on instruments with collinear lasers) |
| Comments | Compatible with green (532 nm) and yellow-green (561 nm) lasers; Tandem dye is sensitive to degradation. Protect from light and avoid prolonged exposure to paraformaldehyde containing fixatives. |