Human Stem Cell Markers

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Embryonic

Surface

Marker Clone Purpose/Comments Violet Laser Blue Laser Red Laser
CD9 HI9a Expressed on ES and neural stem cells. Involved in migration and adhesion. FITC
(2160515, 2160520)PE
(2160515, 2160530)
CD24 ML5 Expressed on ES and neural progenitor cells with a possible role in cell migration. BV5421
(2155605, 2155610)BV510
(2155625, 2155630)BV605
(2155615, 2155620)

FITC
(2155515, 2155520)AF488
(2155540)PerCP
(2155565, 2155570)

PerCP/Cy5.5
(2155575, 2155580)

PE
(2155525, 2155530)

PE/Cy7
(2155595, 2155600)

 APC
(2155585, 2155590)AF647
(2155545, 2155550)
CD30 BY88 Expressed on the surface of induced pluripotent stem cells. FITC
(2269520)PE
(2269530)		 APC
(2269545, 2269550)
CD59 p282(H19) Reported to be expressed on embryonic, mesenchymal, and neural stem cells. FITC
(2123530)PE
(2123535, 2123540)
SSEA-5 8e11 Expressed on the surface of embryonic and pluripotent stem cells. FITC
(2376035, 2376040)AF488
(2376025, 2376030)PE
(2376015, 2376020)		 APC
(2376045, 2370650)

Intracellular

Marker Clone Purpose Violet Laser Blue Laser Red Laser
Nanog* 16H3A48 Transcription factor involved in the propagation of undifferentiated embryonic stem cells.
23D2-3C6
Oct4
(Oct3)		 3A2A20 A transcription factor that regulates pluripotency of embryonic stem cells. A488
(3868525, 3868530)PE
(3868515, 3868520)
SOX2 14A6A34 A transcription factor that regulates pluripotency of embryonic stem cells. A488
(3880545, 3880550)PE
(3880515, 3880520)		 A647
(3880540)

Hematopoetic

Surface

Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD31 WM59 An adhesion molecule expressed on hematopoetic and mesenchymal stem cells.
CD34 561 Key hematopoetic stem cell marker. BV421
(2318045, 2318050)		 FITC
(2318015, 2318020)PE
(2318025, 2318030)		 APC
(2318035, 2318040)
581 PacBlue
(2317555, 2317560)BV510
(2317635)

FITC
(2317515, 2317520)AF488
(2317585, 2317590)PerCP
(2317595, 2317600)

PerCP/Cy5.5
(2317605, 2317610)

PE
(2317525, 2317530)

PE/Cy7
(2317575, 2317580)

APC
(2317545, 2317550)AF647
(2317535, 2317540)AF700
(2317625, 2317630)

APC/Cy7
(2317565, 2317570)
CD49f GoH3 Potential hematopoetic and cancer stem cell marker. PacBlue
(2168095, 2168100)BV421
(2168115, 2168120)

FITC
(2168025, 2168030)AF488
(2168035, 2168040)PerCP/Cy5.5
(2168085, 2168090)

PE
(2168055, 2168060)

PE/Cy7
(2168105, 2168110)

 APC
(2168075, 2168080)AF647
(2168045, 2168050)
CD90 5E10 Marker present on many kinds of stem cells. BV421
(2240605, 2240610)BV510
(2240625)BV605
(2240635, 2240640)

FITC
(2240535, 2240540)PerCp/Cy5.5
(2240585, 2240590)PE
(2240545, 2240550)

PE/Cy5
(2240555, 2240560)

PE/Cy7
(2240615, 2240620)

 APC
(2240565, 2240570)AF647
(2240575, 224580)AF700
(2240595, 2240600)
CD17(c-KIT) 104D2 Hematopoetic and cancer stem cell marker.

BV421
(2166075, 2166080)BV510
(2166095, 2166100)BV605
(2166085, 2166090)

BV650
(2166105)

PerCP/Cy5.5
(2166065, 2166070)PE
(2166015, 2166020)PE/Cy5
(2166050)

PE/Cy7
(2166050, 2166060)

 APC
(2166025, 2166030)
A3C6E2

PerCP/Cy5.5
(2217080)PE
(2217035, 2217040)PE/Cy5
(2217060)

PE/Cy7
(2217070)
CD166 3A6 Potential hematopoetic and cancer stem cell marker. PE
(2319515, 2319520)

Mesenchymal(MSC)

Surface

Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD10 HI10a Expressed on MSC and involved in cell signaling. BV421
(2161085, 2161090)BV510
(2161095, 2161100)BV605
(2161105, 2161110)

FITC
(2161035, 2161040)PerCP/Cy5.5
(2161075, 2167080)PE
(2161015, 2161020)

PE/Cy5
(2161030)

PE/Cy7
(2161065, 2161070)

 APC
(2161045, 2161050)APC/Cy7
(2161060)
CD13 WM15 Cell surface peptidase expressed on MSC. BV421
(2108575, 210858)		 PE
(2108515, 2108520)PerCP/Cy5.5
(2108565, 2108570)PE/Cy7
(2108555, 2108560)		 APC
(2108525, 2108530)APC/Cy7
(2108545, 2108550)
CD31 WM59 An adhesion molecule expressed on hematopoetic and MSC. PacBlue
(2115565, 2115570)BV421
(2115615, 2115620)Bv605
(2115605, 2115610)

FITC
(2115515, 2115520)AF488
(2115545, 2115550)PE
(2115525, 2115530)

PE/Cy7
(2115585, 2115590)

 APC
(2115575, 2115580)AF647
(2115555, 2115560)APC/Cy7
(2115595, 21155600)
CD44 BJ18 Mesenchymal and cancer stem cell marker. BV421
(2294045, 2294050)		 FITC
(2294015, 2294020)PE
(2294035, 2294040)		 APC
(2294025, 2294030)
IM7

PacBlue
(1115095, 1115100)BV421
(1115195)BV510
(1115215)

BV570
(1115185)

BV605
(1115235)

BV650
(1115245)

BV711
(1115245)

BV785
(1115205)

FITC
(1115105, 1115110)AF488
(1115075, 1115080)PerCP
(1115175, 1115180)

PerCP/Cy5.5
(1115155, 1115160)

PE
(1115115, 1115120)

PE Dazzle 594
(1115275, 1115280)

PE/Cy5
(1115045, 1115050)

PE/Cy7
(1115145, 1115150)

APC
(1115055, 1115060)AF647
(1115085, 1115090)AF700
(1115125, 1115130)

APC/Cy7
(1115135, 1115140)
CD50 CBR-IC3/1 Marker present on MSC. PE
(2250025)		 APC
(2250055)
CD59 p282(H19) Reported to be expressed on embryonic, mesenchymal, and neural stem cells FITC
(2123530)PE
(2123535, 2123540)
CD73 AD2 Key mesenchymal stem cell marker. PE/Dazzle
(2320095, 2320100)
CD90 5E10 Marker present on many kinds of stem cells. BV421
(2240605, 2240610)BV510
(2240625)BV605
(2240635, 2240640)

FITC
(2240535, 2240540)PerCp/Cy5.5
(2240585, 2240590)PE
(2240545, 2240550)

PE/Cy5
(2240555, 2240560)

PE/Cy7
(2240615, 2240620)

 APC
(2240565, 2240570)AF647
(2240575, 224580)AF700
(2240595, 2240600)
CD105 43A3 Mesenchymal stem cell marker with a role in angiogenesis. BV421
(2216095)

FITC
(2216015, 2216020)AF488
(2216045, 2216050)PerCP/Cy5.5
(2216075, 2216080)

PE
(2216025, 2216030)

PE/Cy7
(2216085, 2216090)

 APC
(2216035, 2216040)AF647
(2216060)
CD106 STA An adhesion molecule expressed on mesenchymal stem cells. PE
(2129025, 2129030)PE/Cy5
(2129030)		 APC
(2129045, 2129050)
CD146 P1H12 An adhesion molecule expressed on mesenchymal stem cells. BV421
(2405105)

FITC
(2405055, 2405060)PerCP/Cy5.5
( 2405045, 2405050)PE
(2405025, 2405030)

PE/Cy7
(2405035, 2405040)

 APC
(2405075, 2405080)AF647
(2405065, 2405070)
SHM-57

AF488
(2310035, 2310040)PerCP/Cy5.5
(2310065, 2310070)PE
(2310015, 2310020)

PE/Cy7
(2310045, 2310050)

 APC
(2310055, 2310060)AF647
(2310025, 2310030)
CD166 3A6 Mesenchymal stem cell marker with a role in cell migration and adhesion. PE
(2319515, 2319520)
Stro-1 STRO-1 Mesechymal stem cell marker with a potential role in cell migration. FITC
(2300525, 2300530)		 AF647
(2300515, 2300520)

Neural

Extracellular

Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD9 HI9a Expressed on ES and neural stem cells. Involved in migration and adhesion. FITC
(2160515, 2160520)PE
(2160515, 2160530)
CD13 WM15 Cell surface peptidase expressed on MSC and neural progenitor cells. BV421
(2108575, 2108580)		 PerCp/Cy5.5
(2108565, 2108570)PE
(2108515, 2108520)PE/Cy7
(2108555, 2108560)		 APC
(2108525, 2108530)APC/Cy7
(2108545, 2108550)
CD15(SSEA-1) HI98 A neural stem cell marker with a role in cell adhesion. FITC
(2109515, 2109520)AF488
(2109550)PE
(2109525, 2109530)		 APC
(2109535, 2109540)
W6D3

PacBlue
(2215105, 2215110)BV510
(2215135, 2215140)BV605
(2215155, 2215160)

BV650
(2215165)

FITC
(2215015, 2215020)AF488
(2215050)PerCP
(2215090)

PerCP/Cy5.5
(2215095, 2215100)

PE
(2215025, 2215030)

PE Dazzle 594
(2215185, 2215190)

PE/Cy5
(2215065, 2215070)

PE/Cy7
(2215145, 2215150)

 APC
(2215035, 2215040)AF647
(2215060)AF700
(2215130)
CD24 ML5 Marker for maturing neuronal cell types. BV5421
(2155605, 2155610)BV510
(2155625, 2155630)BV605
(2155615, 2155620)

FITC
(2155515, 2155520)AF488
(2155540)PerCP
(2155565, 2155570)

PerCP/Cy5.5
(2155575, 2155580)

PE
(2155525, 2155530)

PE/Cy7
(2155595, 2155600)

 APC
(2155585, 2155590)AF647
(2155545, 2155550)
CD56 1.1H11 Cell adhesion marker expressed on neural cells. PacBlue
(2412595, 2412600)BV650
(2412655, 2412660)

AF488
(2412585, 2412590)PerCP
(2412625, 2412630)PerCP/Cy5.5
(2412525, 2412530)

PE
(2412535, 2412540)

PE/Cy5
(2412575, 2412580)

PE/Cy7
(2412545, 2412550)

APC
(2412515, 2412520)AF647
(2412565, 2412570)AF700
(2412605, 2412610)

APC/Cy7
(2412555, 2412560)
HCD56

PacBlue
(2191625, 2191630)BV421
(2191635, 2191640)BV510
(2191695, 2191700)

BV570
(2191645, 2191670)

BV605
(2191665, 2191670)

BV650
(2191715)

BV711
(2191675, 2191680)

FITC
(2191515, 2191520)AF488
(2191555, 2191560)PerCP
(2191705, 2191710)

PerCP/Cy5.5
(2191605, 2191610)

PE
(2191525, 2191530)

PE Dazzle
(2191735, 2191740)

PE/Cy5
(2191540)

PE/Cy7
(2191585, 2191590)

APC
(2191545, 2191550)AF647
(2191565, 2191570)AF700
(2191575, 2191580)

APC/Cy7
(2191655, 2191660)
MEM-188 PacBlue
(2123145)

FITC
(2123015, 2123020)AF488
(2123055)PerCP/Cy5.5
(2123125, 2123130)

PE
(2123025, 2123030)

PE/Cy5
(2123035, 2123040)

PE/Cy7
(2123140)

 APC
(2123050)AF647
(2123060)
CD59 p282(H19) Reported to be expressed on embryonic, mesenchymal, and neural stem cells. FITC
(2123530)PE
(2123535, 2123540)
CD138 1D4 Proposed neural stem cell marker PE
(2645015, 2645020)
MI15

BV421
(2382575, 2382580)BV510
(2382585, 2352590)BV605
(2382595, 2382600)

BV711
(2382605, 2382610)

FITC
(2382535, 2382540)PerCP/Cy5.5
(2382545, 2382550)PE
(2382515, 2382520)

PE/Cy7
(2382565, 2382570)

 APC
(2382525, 2382530)AF647
(2382615, 2382620)AF700
(2382555, 2382560)
CD184 12G5 Expressed on neural and MSC. Involved in cell migration. BV421
(2132585, 2132590)BV605
(2132605, 2132610)

PerCP/Cy5.5
(2132575, 2132580)PE
(2132525, 2132530)PE/Dazzle 594
(2312625, 2132630)

PE/Cy5
(2132535, 2132540)

PE/Cy7
(2132565, 2132570)

 APC
(2132545, 213255)

Intracellular

Marker Clone Purpose Violet Laser Blue Laser Red Laser
SOX2 14A6A34 Transcription factor involved in the maintenance of neural stem cell properties. A488
(3880545, 3880550)PE
(3880515, 3880520)		 A647
(3880540)

Cancer Stem Cell markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD44 BJ18 Potential cancer stem cell marker. BV421
(2294045, 2294050)		 FITC
(2294015, 2294020)PE
(2294035, 2294040)		 APC
(2294025, 2294030)
IM7

PacBlue
(1115095, 1115100)BV421
(1115195)BV510
(1115215)

BV570
(1115185)

BV605
(1115235)

BV711
(1115285)

BV785
(1115205)

FITC
(1115105, 115110)AF488
(1115075, 1115080)PerCP
(1115175, 1115180)

PerCP/Cy5.5
(1115155, 1115160)

PE
(1115115, 1115120)

PE Dazzle
(1115275, 1115280)

PE/Cy5
(1115045, 1115050)

PE/Cy7
(1115145, 1115150)

APC
(1115055, 1115060)AF647
(1115085, 1115090)AF700
(1115125, 1115130)

APC/Cy7
(1115135, 1115140)
CD49f GoH3 Potential hematopoetic and cancer stem cell marker. PacBlue
(2168095, 2168100)BV421
(2168115, 2168120)

FITC
(2168025, 2168030)AF488
(2168035, 2168040)PerCP/Cy5.5
(2168085, 2168090)

PE
(2168055, 2168060)

PE/Cy7
(2168105, 2168110)

 APC
(2168075, 2168080)AF647
(2168045, 2168050)
CD90 5E10 Potential cancer stem cell marker; Stem cell progenitor marker. BV421
(2240605, 2240610)BV510
(2240625, 2240630)BV605
(2240640)

FITC
(2240535, 2240540)PerCp/Cy5.5
(2240585, 2240590)PE
(2240545, 2240550)

PE/Cy5
(2240555, 2240560)

PE/Cy7
(2240615, 2240620)

 APC
(2240565, 2240570)AF647
(2240575, 2240580)AF700
(2240595, 2240600)
CD117 104D2 Germ Cell development and cancer stem cell.

BV421
(2166075, 2166080)BV510
(2166095, 2166100)BV605
(2166085, 2166090)

BV650
(2166105)

PerCP/Cy5.5
(2166065, 2166070)PE
(2166015, 2166020)PE/Cy5
(2166050)

PE/Cy7
(2166050, 2166060)

 APC
(2166025, 2166030)
A3C6E2 PerCP/Cy5.5
(2217080)PE
(2217035, 2217040)PE/Cy5
(2217060)PE/Cy7
(2217070)
CD166 3A6 Potential cancer stem cell marker. PE
(2319515, 2319520)
CD326(EpCAM) 9C4 Potential cancer stem cell marker.

PacBlue
(2221085, 2221090)BV421
(2221095, 2221100)BV605
(2221115, 2221120)

BV650
(2221125, 2221130)

FITC
(2221015, 2221020)AF488
(2221045, 2221050)PerCP/Cy5.5
(2221065, 2221070)

PE
(2221025, 2221030)

PE Dazzle
(2221155, 2221160)

PE/Cy7
(2221105, 2221110)

 AF647
(2221060)
Stro-1 STRO-1 Potential cancer stem cell marker. FITC
(2300525, 2300530)		 AF647
(2300515, 2300520)

Mesenchymal Stem Cell Panel

Specificity Clone Fluorochrome Purpose Cat. No. Name
CD73 AD2 PE/Dazzleâ„¢ 594 Identification of mesenchymal stem cells 2320095 PE/Dazzleâ„¢ 594 Anti-human CD73 (Ecto-5'-nucleotidase) Antibody
CD90 5E2 Alexa Fluor® 647 Identification of mesenchymal stem cells 2240575 Alexa Fluor® 647 Anti-human CD90 (Thy1) Antibody
CD105 43A3 Brilliant Violet 421â„¢ Identification of mesenchymal stem cells 2216095 Brilliant Violet 421â„¢ Anti-human CD105 Antibody
CD14 M5E2 Brilliant Violet 711â„¢ Negative marker 2109185 Brilliant Violet 711â„¢ Anti-human CD14 Antibody
CD45 HI30 APC/Cy7 Negative marker 2120070 APC/Cy7 Anti-human CD45 Antibody
CD34 561 PE Negative marker 2318025 PE Anti-human CD34 Antibody
CD19 HIB19 PerCP/Cy5.5 Negative marker 2111145 PerCP/Cy5.5 Anti-human CD19 Antibody
HLA-DR L243 Alexa Fluor® 488 Negative marker 2138095 Alexa Fluor® 488 Anti-human HLA-DR Antibody

Breast Cancer Stem Cell

Specificity Clone Fluorochrome Purpose Cat. No. Name
CD44 IM7 PE Identification of cancer stem cells 1115115 Anti-mouse/human CD44 Antibody
CD24 ML5 Alexa Fluor® 647 Usually a low/negative marker for breast cancer stem cells 2155545 Alexa Fluor® 647 Anti-human CD24 Antibody