Flow Cytometry Reagents for Cancer Research - Beyond Annexin V

The Annexin V assay which measures exposed phosphatidyl serines, a early marker of apoptosis, is a well established assay for cancer research. Using flow cytometry, apoptotic cells can be distinguished from dead cells, with a semi-quantitiative read-out. With the ability to measure multiple parameters simulataneously, flow cytometry is a particularly valuable tool when samples are limited. In addtion to apoptosis, cell prolifertation and DNA damage can be easily measured within the same sample through BrdU incorporation and H2AX.

Flow cytometry is also a practical tool for the study of the immune system in cancer. The discovery of markers such as PD-L1 (CD274) whose expression is linked to immune evasion by tumors, have re-invigorated the area of tumor immunology.

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Markers for Apoptosis, DNA Damage and Cell Proliferation

Marker Clone Purpose/Comments Violet Laser Blue Laser Red Laser
Annexin V N/A Apoptosis detection PacBlue
(3804585, 3804590)BV421
(3804615)BV510
(3804685)		 FITC
(3804525, 3804530)PerCP/Cy5.5
(3804675, 3804680)PE
(3804535, 3804540)		 APC
(3804595, 3804600)AF647
(3804555, 3804560)
BrdU 3D4 Proliferation. BrdU is pulsed into cells and then measured using this antibody AF488
(2420525, 2420530)
BrdU Bu20a PE
(2299055, 2299060)		 APC
(2299035)
H2A.X-Phosphorylated
(Ser139)		 2F3 DNA damage measurement FITC
(3667015, 3667020)AF488
(3667025, 3667030)		 AF647
(3667035, 3667040)
Ki67 Ki-67 Cell proliferation

BV421
(2352525, 2352530)BV510
(2352585)BV605
(2352605, 2352610)

BV711
(2352575, 2352580)

AF488
(2352535, 2352540)PerCP/Cy5.5
(2352595, 2352600)PE
(2352515, 2352520)

PE/Cy7
(2352625, 2352630)

 AF700
(2352645, 2352650)
Marker Clone Purpose Violet Laser Blue Laser Red Laser
Bcl-2 100 Tumor suppression BV421
(3893545)		 AF488
(3893515, 3893520)PE
(3893535, 3893540)		 AF647
(3893525, 3893530)
Bcl-2 BCL/10C4 FITC
(3767515, 3767520)AF488
(3767525, 3767530)PE
(3767535, 3767540)		 AF647
(3767545, 3767550)
p53 DO-7 Tumor suppression FITC
(3829015, 3829020)PE
(3829025, 3829030)

Cancer Immunology Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD3 HIT3a T-cell lineage marker PacBlue
(2101645, 2101650)

FITC
(2101525, 2101530)AF488
(2101595, 2101600)PerCP/Cy5.5
(2101635, 2101640)

PE
(2101535, 2101540)

PE/Cy5
(2101545, 2101550)

PE/Cy7
(2101580)

APC
(2101555, 2101560)AF647
(2101605, 2101610)AF700
(2101615, 2101620)

APC/Cy7
(2101585, 2101590)
OKT3

PacBlue
(2186565, 2186570)BV510
(2186655, 2186660)BV605
(2186605, 2186610)

BV650
(2186615, 2186620)

BV711
(2186635, 2186640)

BV785
(2186645, 2186650)

FITC
(2186525, 2186530)AF488
(2186550)PerCP
(2186685, 2186690)

PerCP/Cy5.5
(2186675, 2186680)

PE
(2186535, 2186540)

PE/Cy7
(2186665, 2186670)

APC
(2186585, 2186590)AF647
(2186560)AF700
(2186695, 2186700)

APC/Cy7
(2186705, 2186710)
SK7 PacBlue
(2324115, 2324120)BV510
(2324135, 2324140)

FITC
(2324015, 2324020)AF488
(2324050)PerCP
(2324065, 2324070)

PerCP/Cy5.5
(2324035, 2324040)

PE
(2324025, 2324030)

PE/Cy7
(2324075, 2324080)

APC
(2324055, 2324060)AF647
(2324125, 2324130)AF700
(2324105, 2324110)

APC/Cy7
(2324085, 2324090)
UCHT1

PacBlue
(2102085, 2102090)BV421
(2102165, 2102170)BV510
(2102235, 2102240)

BV570
(2102175, 2102180)

BV605
(2102295, 2102300)

FITC
(2102025, 2102030)AF488
(2102075)PerCP
(2102135, 2102140)

PerCP/Cy5.5
(2102145, 2102150)

PE
(2102035, 2102040)

PE Dazzle 594
(2102245, 2102250)

PE/Cy5
(2102050)

PE/Cy7
(2102095, 2102100)

APC
(2102055, 2102060)AF647
(2102110, 2102080)AF700
(2102115, 2102120)

APC/Cy7
(2102125, 2102130)
CD8 SK1 Cytotoxic T Cell PacBlue
(2323585, 2323590)

FITC
(2323515, 2323520)AF488
(2323580)PerCP
(2323535, 2323540)

PerCP/Cy5.5
(2323545, 2323550)

PE
(2323525, 2323530)

PE/Cy7
(2323555, 2323560)

APC
(2323605, 2323610)AF647
(2323625, 2323630)AF700
(2323615, 2323620)

APC/Cy7
(2323565, 2323570)
CD19 HIB19 B-cell lineage marker

PacBlue
(2111115, 2111120)BV421
(2111165, 2111170)BV510
(2111205, 2111210)

BV570
(2111175, 2111180)

BV605
(2111215, 2111220)

BV650
(2111185, 2111190)

BV711
(2111225, 2111230)

BV785
(2111195, 2111200)

FITC
(2111025, 2111030)AF488
(2111095)PerCP
(2111135, 2111140)

PerCP/Cy5.5
(2111145, 2111150)

PE
(2111035, 2111040)

PE Dazzle 594
(2111255, 2111260)

PE/Cy5
(2111045, 111050)

PE/Cy7
(2111075, 2111080)

APC
(2111055, 2111060)AF647
(2111110, 2111115)AF700
(2111125, 2111130)

APC/Cy7
(2111085, 2111090)
SJ25C1

BV421
(2415085, 2415090)BV510
(2415095, 2415100)BV605
(2415115, 2415120)

BV650
(2415125, 2415130)

BV711
(2415105, 2415110)

BV785
(2415135, 2415140)

FITC
(2415035, 2415040)PerCP
(2415065, 2415070)PerCP/Cy5.5
(2415075, 2415080)

PE
(2415015, 2415020)

PE/Cy7
(2415055, 2415060)

 APC
(2415025, 2415030)APC/Cy7
(2415045, 2415050)
CD20 2H7 Expressed on all B cells with levels increasing through B cell maturity

PacBlue
(2111595, 2111600)BV421
(2111695, 2111650)BV510
(2111695, 2111700)

BV570
(2111655)

BV605
(2111665, 2111670)

BV650
(2111675, 2111680)

BV711
(2111705, 2111710)

FITC
(2111515, 2111520)AF488
(2111580)PerCP
(2111615, 2111620)

PerCP/Cy5.5
(2111625, 2111630)

PE
(2111525, 2111530)

PE Dazzle 594
(2111735, 2111740)

PE/Cy5
(2111535, 2111540)

PE/Cy7
(2111555, 2111560)

APC
(2111545, 2111550)AF647
(2111590)AF700
(2111610)

APC/Cy7
(2111565, 2111570)
CD24 ML5 Potential cancer stem cell marker BV421
(2155605, 2155610)BV510
(2155625, 2155630)BV605
(2155615, 2155620)

FITC
(2155515, 2155520)AF488
(2155540)PerCP
(2155565, 2155570)

PerCP/Cy5.5
(2155575, 2155580)

PE
(2155525, 2155530)

PE/Cy7
(2155595, 2155600)

 APC
(2155585, 2155590)AF647
(2155545, 2155550)
CD34 561 Hematopoetic stem cell marker BV421
(2318045, 2318050)		 FITC
(2318015, 2318020)PE
(2318025, 2318030)		 APC
(2318035, 2318040)
581 PacBlue
(2317555, 2317560)BV510
(2317635)

FITC
(2317515, 2317520)AF488
(2317585, 2317590)PerCP
(2317595, 2317600)

PerCP/Cy5.5
(2317605, 2317610)

PE
(2317525, 2317530)

PE/Cy7
(2317575, 2317580)

APC
(2317545, 2317550)AF647
(2317535, 2317540)AF700
(2317625,2317630)

APC/Cy7
(2317565, 2317570)
CD38 HB-7 B-cell differentiation marker BV510
(2383055, 2383060)

FITC
(2383045, 2383050)PerCP/Cy5.5
(2383065, 2383070)PE
(2383015, 2383020)

PE/Cy7
(2383035, 2383040)

 APC
(2383025, 2383030)APC/Cy7
(2383075, 2383080)
HIT2 BV421
(2117625, 2117630)BV605
(2117655, 2117660)BV711
(2117635, 2117640)

FITC
(2117515, 2117520)AF488
(2117555, 2117560)PerCP
(2117595, 2117600)

PerCP/Cy5.5
(2117605, 2117610)

PE
(2117525, 2117530)

PE Dazzle 594
(2117685, 2117690)

PE/Cy5
(2117535, 2117540)

PE/Cy7
(2117575, 2117580)

APC
(2117545, 2117550)AF647
(2117570)AF700
(2117615, 2117620)

APC/Cy7
(2117665, 2117670)
CD44 BJ18 Potential cancer stem cell marker BV421
(2294045, 2294050)		 FITC
(2294015, 2294020)PE
(2294035, 2294040)		 APC
(2294025, 2294030)
IM7

PacBlue
(1115095, 1115100)BV421
(1115195)BV510
(1115215)

BV570
(1115185)

BV605
(1115235)

BV650
(1115245)

BV711
(1115245)

BV785
(1115205)

FITC
(1115105, 1115110)AF488
(1115075, 1115080)PerCP
(1115175, 1115180)

PerCP/Cy5.5
(1115155, 1115160)

PE
(1115115, 1115120)

PE Dazzle 594
(1115275, 1115280)

PE/Cy5
(1115045, 1115050)

PE/Cy7
(1115145, 1115150)

APC
(1115055, 1115060)AF647
(1115085, 1115090)AF700
(1115125, 1115130)

APC/Cy7
(1115135, 1115140)
CD90 5E10 Potential cancer stem cell marker BV421
(2240605, 2240610)BV510
(2240625)BV605
(2240635, 2240640)

FITC
(2240535, 2240540)PerCP/Cy5.5
(2240585, 2240590)PE
(2240545, 2240550)

PE/Cy5
(2240555, 2240560)

PE/Cy7
(2240615, 2240620)

 APC
(2240565, 2240570)AF647
(2240575, 2240580)AF700
(2240595, 2240600)
CD105 43A3 Endothelial marker useful for the study of angiogenesis BV421
(2216095)

FITC
(2216015, 2216020)AF488
(2216045, 2216050)PerCP/Cy5.5
(2216075, 2216080)

PE
(2216025, 2216030)

PE/Cy7
(2216085, 2216090)

 APC
(2216035, 2216040)AF647
(2216060)
CD166 3A6 Potential cancer stem cell marker PE
(2319515, 2319520)
CD274
(B7-H1, PD-L1)		 29E.2A3 Expressed on many tumor cells. Helps tumor cells evade the immune system BV521
(2248565, 2248570)		 PE
(2248525, 2248530)PE/Cy7
(2248585, 2248590)		 APC
(2248535, 2248540)
CD279
(PD-1)		 EH12.2H7 Cancer immune evasion

PacBlue
(2249575, 2249580)BV421
(2249595, 2249600)BV510
(2249655, 2249660)

BV605
(2249615, 2249620)

BV711
(2249635, 2249640)

BV785
(2249645, 2249650)

FITC
(2249515, 2249520)AF488
(2249675, 2249680)PerCP
(2249685, 2249690)

PerCP/Cy5.5
(2249565, 2249570)

PE
(2249525, 2249530)

PE Dazzle 594
(2249695, 2249700)

PE/Cy7
(2249585, 2249590)

 APC
(2249535, 2249540)AF647
(2249550)APC/Cy7
(2249605, 2249610)
CD326
(EpCAM)		 9C4 Potential cancer stem cell marker

PacBlue
(2221085, 2221090)BV421
(2221095, 2221100)BV605
(2221115, 2221120)

BV650
(2221125, 2221130)

FITC
(2221015, 2221020)AF488
(2221045, 2221050)PerCP/Cy5.5
(2221065, 2221070)

PE
(2221025, 2221030)

PE Dazzle 594
(2221155, 2221160)

PE/Cy7
(2221105, 2221110)

 APC
(2221035, 2221040)AF647
(2221060)
Stro-1 STRO-1 Potential cancer stem cell marker FITC
(2300525, 2300530)		 AF647
(2300515, 2300520)

Basic Treg Markers

Marker Clone Purpose Violet Laser Blue Laser Red Laser
CD4 A161A1 T helper cell marker FITC
(2387025, 2387030)PE
(2387015, 2387020)
OKT4

PacBlue
(2187115, 2187120)BV421
(2187165, 2187170)BV510
(2187215, 2187220)

BV570
(2187225)

BV605
(2187185, 2187190)

BV650
(2187175)

BV711
(2187195, 2187200)

BV785
(2181205, 2187210)

FITC
(2187035, 2187040)AF488
(2187095, 2187100)PerCP
(2187155, 2187160)

PerCP/Cy5.5
(2187135, 2187140)

PE
(2187045, 2187050)

PE/Cy5
(2187055, 187060)

PE/Cy7
(2187065, 2187070)

APC
(2187075, 2187080)AF647
(2187110)AF700
(2187125, 2187130)

APC/Cy7
(2187085, 2187090)
RPA-T4

PacBlue
(2102620, 2102605)BV421
(2102655, 2102660)BV510
(2102725, 2102730)

BV570
(2102665, 2102670)

BV605
(2102775, 2102780)

BV650
(2102675)

BV711
(2102785, 2102790)

BV785
(2102765, 2102770)

FITC
(2102525, 2102530)AF488
(2102595)PerCP
(2102635, 2102640)

PerCP/Cy5.5
(2102645, 2102650)

PE
(2102535, 2102540)

PE Dazzle 594
(2102735, 2102740)

PE/Cy5
(2102545, 2102550)

PE/Cy7
(2102555, 2102560)

APC
(2102570)AF647
(2102615, 2102600)AF700
(2102630)

APC/Cy7
(2102585, 2102590)
SK3 PacBlue
(2323095, 2323100)

FITC
(2323020)AF488
(2323090)PerCP
(2323115, 2323120)

PerCP/Cy5.5
(2323035, 2323040)

PE
(2323025, 2323030)

PE/Cy7
(2323055, 2323060)

 APC
(2323065, 2323070)AF700
(2323105, 2323110)APC/Cy7 2323075, 2323080)
CD25 BC96 Expressed at high levels on Tregs.

PacBlue
(2113135)BV421
(2113145, 2113150)BV605
(2113155, 2113160)

BV650
(2113165, 2113170)

BV700
(2113175, 2113180)

BV785
(2113185)

FITC
(2113015, 2113020)AF488
(2113075, 2113080)PerCP/Cy5.5
(2113125, 2113130)

PE
(2113025, 2113030)

PE/Cy5
(2113035, 2113040)

PE/Cy7
(2113055, 2113060)

APC
(2113045, 2113050)AF647
(2113085, 2113090)AF700
(2113105, 2113110)

APC/Cy7
(2113065, 2113070)
M-A251 PacBlue
(2380645, 2380650)BV421
(2380565, 2380570)BV510
(2380595, 2380600)

FITC
(2380525, 2380530)AF488
(2380575, 2380580)PerCP
(2380655, 2380660)

PerCP/Cy5.5
(2380555, 2380560)

PE
(2380515, 2380520)

PE Dazzle 594
(2380625, 2380630)

PE/Cy7
(2380535, 2380540)

APC
(2380545, 2380550)AF647
(2380635, 2380640)AF700
(2380585, 2380590)

APC/Cy7
(2380605, 2380610)
CD127
(IL-7Rα)		 A019D5 Inversely expressed with FoxP3. Negative expression is used for Treg sorting.

PacBlue
(2356525, 2356530)BV421
(2356545, 2356550)BV510
(2356655, 2356660)

BV570
(2356535)

BV605
(2356665, 2356670)

BV650
(2356625, 2356630)

BV711
(2356635, 2356640)

BV785
(2356645, 2356650)

FITC
(2356555, 2356560)AF488
(2356565, 236570)PerCP/Cy5.5
(2356605, 2356610)

PE
(2356515, 256520)

PE Dazzle 594
(2356675, 2356680)

PE/Cy5
(2356615, 2356620)

PE/Cy7
(2356595, 2356600)

 APC
(2356575, 2356580)AF647
(2356585, 2356590)
CD194 (CCR4) L291H4 T Reg homing marker BV421
(2397065, 2397070)BV510
(2397075, 2397080)BV605
(2397085, 2397090)

PerCP/Cy5.5
(2397025, 2397030)PE
(2397055, 2397060)PE Dazzle 594
(2397095, 2397100)

PE/Cy7
(2397045, 2397050)

 APC
(2397035, 2397040)AF647
(2397015, 2397020)
TG6/CCR4 PerCP/Cy5.5
(2277015)		 AF647
(2277005)

Kits for Apoptosis Detection

Name Fluorochromes Kit Contents Cat. No
APC Annexin V Apoptosis Detection Kit with 7-AAD APC APC Annexin V, 7-AAD Viability Staining Solution, Annexin V Binding Buffer 3804650
FITC Annexin V Apoptosis Detection Kit with 7-AAD FITC FITC Annexin V, 7-AAD Viability Staining Solution, Annexin V Binding Buffer 3804610
Pacific Blueâ„¢ Annexin V Apoptosis Detection Kit with 7-AAD Pacific Blueâ„¢ Pacific Blueâ„¢ Annexin V, 7-AAD Viability Staining Solution, Annexin V Binding Buffer 3804630
PE Annexin V Apoptosis Detection Kit with 7-AAD PE PE Annexin V, 7-AAD Viability Staining Solution, Annexin V Binding Buffer 3804670
APC Annexin V Apoptosis Detection Kit with PI APC APC Annexin V, Propidium Iodide Solution, Annexin V Binding Buffer 3804660
FITC Annexin V Apoptosis Detection Kit with PI FITC FITC Annexin V, Propidium Iodide Solution, Annexin V Binding Buffer 3804570
Pacific Blueâ„¢ Annexin V Apoptosis Detection Kit with PI Pacific Blueâ„¢ Pacific Blueâ„¢ Annexin V, Propidium Iodide Solution, Annexin V Binding Buffer 3804640

Breast Cancer Stem Cell

Specificity Clone Fluorochrome Purpose Cat. No. Name
CD44 IM7 PE Identification of cancer stem cells 1115115 Anti-mouse/human CD44 Antibody
CD24 ML5 Alexa Fluor® 647 Usually a low/negative marker for breast cancer stem cells 2155545 Alexa Fluor® 647 Anti-human CD24 Antibody