- Sony Biotechnology
- Sony Biotechnology
Available On Demand—Originally presented on Thursday, March 14, 2019 by Dr. Peter Lopez and Dr. Rui Gardner This SelectScience® webinar will be valuable to those with any level of cell sorting experience who use flow cytometry and want to improve their experimental outcomes. This session addresses three key considerations for effective cell sorting. You’ll learn: How purity, efficiency, and speed influence good sorting outcomes—and choices you’ll need to make to optimize sorting for your application. How instrument characteristics can influence cell sorting performance—and how to select what will work best for your experiment. How to set up for plate sorting to improve single cell deposition efficiency View On Demand →
The Sony MA900 Multi-Application Cell Sorter has been nominated for the Best New Life Sciences Product of 2018! Scientists worldwide are voting in this year's Scientists' Choice Awards® for the best product, and we are thrilled that the MA900 is being recognized as a game changer in the industry. The MA900 is packed with innovative, modern capabilities including 12 fluorescence parameters and 4-way sorting, providing choice and flexibility for most flow cytometry tests and applications. Advanced automation that makes sorting less subjective and more reliable. Sensors and software to dramatically simplify aseptic cleaning, QC, sorting, and troubleshooting. Intelligent and adaptable to meet your needs. Choose from eight default and five custom sort modes to achieve desired purity and yield. Easy to learn and use. Easy-to-use wizards guide users through workflows from start to finish, so researchers can focus more time on science. ✔️ Please click here to vote for the MA900 today!
Gene expression profiling is a powerful approach that promotes a deeper understanding of the characteristics of cells at the transcriptional level and has broad implications for basic and clinical research. Historically, gene expression profiling has been performed on populations of cells, typically cell lysates, where observed expression levels represent an average of the unique expression states of each cell within the population. Such phenotypically similar populations can be purified in bulk by flow cytometry based cell sorting prior to RNA analysis. 1,2
Much of cell research happens in the context of cultured or modified cells. More biologically relevant experiments can be done with blood. However, blood cannot entirely capture what takes place within a live organism. In their paper Futamura, et al. describes a method to study the movement and subsequent interactions of immune cells in live mice by flow cytometry.
Recent advances in genome editing and the application of fluorescent proteins have accelerated Interest in isolating specific populations of brain cells from mixed populations. Researchers are also using cell sorting to isolate single cells for expansion and analysis.
Placing several fluorescent proteins together in a flow cytometry panel offers greater power and capability for experiments. However, handling autofluorescent signal with fluorescent proteins is out of reach for conventional flow cytometetry users. Sony spectral flow cytometry analyzers enable researchers to harness up to five near infra-red fluorescent proteins in a single experiment. Moreover, spectral technology lets users accurately identify autofluorescence, and eliminate it if needed.
Bacteria, most notably the gut microbiome, have gained increased attention for their role in agriculture and human disease. However study of individual bacterium can be challenging since most bacteria do not grow under simple culture conditions. In a recent study, Blow, et al. have published a draft sequence of the bacteria, Candidatus Erwinia dacicola (Enterobacteriaceae). This bacteria has a symbiotic relationship with the Bactrocera oleae, also known as the olive fly. The larva of the olive fly grow in unripened olives leading to reduced crop yields. The larva has adapted to the special environment of the unripened olive with help from Candidatus Erwinia dacicola bacteria. Without bacteria the larva do not develop.
1. Purchase larger sizes of common antibodies on established fluorochromes. Packaging and shipping antibodies can be costly. Therefore smaller sizes typically cost greater than two times more compared to larger sizes.
Bacteria such as E. coli are popular model systems for engineering and production of modified proteins. Yet the idea of putting bacteria in a cell sorter conjures unwelcome images. Take heart, the Sony SH800 cell sorter, simplifies decontamination by quickly and easily letting researchers replace key components that come in contact with the sample. Here are some examples, and publication citations.
Listen to Dr. Jennifer Doudna, one of the discoverers of CRISPR systems discusses how the new genome engineering technology was discovered.