High Speed Motion Capture, Quantification and Visualization
The Sony SI8000 system is equipped with a phase contrast
microscope, a high-speed charge-coupled device (CCD) video
camera, CO2-incubator and sophisticated easy-to-use software.
Focus and light conditions are automated through the SI8000
software. Video imaging captures activity at a high frame rate
(up to 150 fps) to deliver deep data rich results. Displacement
and magnitude of cellular motion are calculated using a Motion
Vector algorithm developed by Sony for HD television. This
allows researchers to accurately analyze movement of single cell
or cell clusters such as monolayers continuously for extended
periods of time. Precise detection of cellular motion provides
label-free analysis of cardiomyocyte contractility, cellular
migration, and apoptosis assays without the need to infer data
from electrophysiological, impedance, or dye based assays.
The software automatically detects and adjusts focus
based on luminance and contrast conditions. Automation
in the motorized stage allows for high throughput analysis
of multi-well culture plates.
Motion Vector Analysis
High spatial and temporal resolution enables high fidelity detection
of the motion of cells (red arrow). The analysis software searches for
luminance similarity using the block matching method from one frame
to the next to accurately calculate displacement vectors, quantifying
the distance the cells moved for richer and more precise data.
Quantification and Visualization
The SI8000 is label-free so cells can be observed in their natural
state free from outside interactions from potentially toxic dyes
or fluorochromes. This capability is particularly useful for toxicity
and safety pharmacology studies as it allows researchers to
see how cells behave as a drug is introduced. An optional CO2
incubator reliably manages an optimal environmental for
in-vitro cell observation.
The scanning stage controls the X, Y and Z position of the
imaging system for precise and repeatable positioning and
re-positioning, supporting high throughput analysis.
Data analysis software enables researchers to quantify and
visualize cells in motion. Cardiomyocytes can be quantified
using contraction and relaxation speed, average deformation
distance, as well as contraction and relaxation duration. Power
Spectral Density that measures the strength of motion in the
frequency domain is also supported. Graphic outputs include
heat maps of contraction, relaxation, propagation and isochrone
maps that detect abnormalities in cardiac cells as well as cellular
and sub-cellular motion visualization.
In Cardiomyocytes, a beat profile is obtained
from Regions of Interest (ROIs). ROIs can be
positioned over single cells or clusters of
cells. From the beat profile, contraction and
relaxation parameters can be calculated.
A sophisticated Motion Vector algorithm
allows for highly accurate propagation
analysis and quantification for a richer and
deeper understanding of results.
Overlay of the beat profiles showing
contraction (first peak) and relaxation
(second peak). The high speed camera
enables precise detection of both
contraction and relaxation of a beating
cardiomyocyte for more precise indication
of beat parameters.
A series of beat profiles showing
contraction (first peak) and relaxation
(second peak). By measuring both
contraction and relaxation, the SI8000
provides a complete profile of the actual
contraction unlike other systems that
infer contraction parameters from
The sophisticated Motion Vector algorithm yields precise visualization of motion
parameters. Power spectral density (PSD) analysis provides visualization of the speed of
motion in single cells or across cell cultures. PSD is displayed in Cardiomyocytes (left) and
neuron culture (right.)
Quantification of cardiomyocytes contractility for Safety Pharmacology assays
High speed video imaging analyzes cardiomyocyte contraction to
detect subtle changes that can occur in response to compounds. The
system delivers excellent insights for safety pharmacology assays. The
phase contrast microscope and high speed video imaging improves
spatial resolution of cardiomyocytes over systems that use fluorescent
or voltage sensitive dyes while providing precise temporal resolution
similar to electrophysiological assays. High spatial and temporal
resolution offers greater depth of data and more precision for safety
By analyzing the actual contraction of cardiomyocytes, the SI8000 has
the ability to detect subtle changes that may occur in response to a
compound or drug.
The SI8000 supports a variety of beat parameters
including beat rate and interval, contraction and
relaxation velocity, shortening length (displacement),
contraction-relaxation duration, synchronicity of
contraction, propagation of contraction, orientation
of contraction direction.
This data shows simultaneous acquisition of multi-electrode
array recordings (blue trace) and motion imaging (red trace).
The red trace shown an Early After Depolarization (EAD)
phenomenon in response to 100 nM E-4031. The SI8000 can
reliably and accurately detect arrhythmic events such as EADs
offering a true representation of the data.
Easily detect markers of arrhythmia
The SI8000 detects both contraction and relaxation to detect subtle
differences in the beat profile that may be missed by other systems. For
example, if field potential duration is modified by a drug or compound,
the system determines the precise effect a drug or compound has on
contraction, relaxation, or both contraction and relaxation, to deliver more
precise data for analysis.
The graphs below represent the beat profile of stem cell derived
cardio-myocytes in response to reference compounds that are known to
have an influence on field potential duration. By analyzing both contraction
and relaxation, the SI8000 can identify changes in the beat profile even in
the absence of changes in electrophysiology or changes in the field
The data above shows how the application of compounds can modify the beat profile. For example,
(A) shows changes in the beat profile in response to lidocaine, (B) shows the beat profile in response
to E-4031, (C) shows the beat profile in response to Flecainide and (D) to Astemizole. The subtle
changes displayed in beat profiles elucidate the depth of data that can be achieved with the SI8000
offering a deep and precise representation of cardiomyocyte function.